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ȱݲw\ԇ_ϺsʢˎI(y)ɷ޹˾
ԇ
\ \

ȱݲw\ԇ

ȱݲw\ԇ

ˎƷQ

ͨQȱݲw\ԇ(ø“(lin)߷)

ӢQDiagnostic Kit for Antibody to Human Immunodeficiency Virus  (ELISA)

hZƴRenlei Mianyi Quexian Bingdu Kangti Zhenduan Shijihe ( Meilianmianyifa)


bҎ(gu)

96˷/

A(y);

ѪҺĺYRHIVȾo\


EIApԭAķzyA(y)HIVԭķ(yng)׃(ni)y˱˱п-HIVt΢װγɿԭ-w(f)cø˿ԭY(ji)γɿԭ-w-ø˿ԭ(f)(jng)@ɫϵy(tng)@ɫ(j)OD ֵжПoHIVwĴڡ


ҪMɳ

1HIVԭ                   96ס1K

2øY(ji)    12ml1ƿ

3HIV-1Ԍա               1.0ml1ƿ

4HIV-2Ԍա             1.0ml1ƿ

5Ԍ                         1.0ml1ƿ

6sϴҺǰÿƿ1:20ϡ    50ml1ƿ

7@ɫA                        6ml1ƿ

8@ɫB    6ml1ƿ

9KֹҺ                            6ml1ƿ

10z                           2Ƭ

11f                           1


lЧڡ

28ܹⱣ12


mÃx

1΢Һ͝^

237ˮԡ

3Ӌr

4ϴC

5ʎ

6ø˃x z򞲨L450nmL630nm


ӱҪ

1ӱɼҪʂ䣬IJѪg(sh)ռѪҺȫѪƷȷ37o2СrٌѪƷx3000D(zhun)/x10ϣʹѪѪ{OٺѪ鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr

2ӱʹѪѪ{ػEDTAȿӰԇY(ji)

3ӱкBcӰԇY(ji)߶ѪĘӱ]ȫտsѪӱ΢ȾĘӱܕe`ĽY(ji)

4rĘӱ28ol¿ɱһڣLڃ-20ⷴ(f)


z򞷽

1ÿԇO(sh)ԃɿסHIV-1ԌպHIV-2ԌոɿքeꎡԌ100lO(sh)һ׿հ׌׼100lyӱ3760ȥ׃(ni)Ʒ۸ɡ

2ϴ壺

1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעMÿףo510룬ȥ׃(ni)ϴҺĸɣ˷(f)5ĸɡ

2ϴCϴ壺xϴ5εijϴϴҺ(yng)עMÿ_ÿoˮĸ

3ÿ׼øY(ji)100lհ׌տײӣ3730ͬϷϴ(yng)5

4ÿ׼@ɫAҺBҺ50lppʎ37ܹo30

5 ÿ׼KֹҺ50lKֹ(yng)ø˃xx(sh)x 450nmԿհ׿У㣩pL450/630nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)


ֵ

1ԇO(sh)ӋҪHIV-1ԌպHIV-2Ԍƽֵ1.20ԌƽֵС0.10ԌODֵ֮(yng)1.20tԇoЧ

2Ԍx(sh)С0.05r0.05Ӌ

3RֵCut off value=Ԍƽֵ+0.10

4yԇ˱ODֵСRֵtHIVw

5yԇ˱ODֵڻRֵtHIVwԡע⣺ԇԘ˱(yng)ȡMp׏(f)ԇ(f)ԇߑ(yng)ȫ̲zyҎ(gu)HIV_CMд_Cԇ򞣩


zY(ji)Ľጡ

1ԽY(ji)ӱв-HIVӱе-HIVԇеęzy

2ԽY(ji)ӱк-HIVخ(yng)

3ԽY(ji)ĘӱҪM؏(f)ԇֻн(jng)^؏(f)؏(f)ԇԞԽY(ji)ĘӱJHIVԡ؏(f)Y(ji)ԵĘӱ(yng)(jng)^m?sh)ֶMд_J

ԽY(ji)Ęӱ(f)zԽY(ji)(yng)JԘӱ؏(f)ԽY(ji)܌һg(sh)}£

1 ڃxӘɵĽȾ

2 @ɫxȾ

3 ԇ©ɵĽȾ

4 ֵϴȥص


ԇ򞷽ľԡ 

ƷHڙzyѪѪ{-HIVRȱݲȾo\


a(chn)Ʒָˡ

1zyHIVwԅƷ(yng)Ҫ

2zyHIVwԅƷ(yng)Ҫ

36HIVw͙zޅƷԷ(yng)3һ|(zh)Ѫ

41HIVwҾԅƷMЙzCV(%)(yng)15%(n=10)

5(wn)Ԍ򞣺ԇM376zY(ji)(yng)_˜ʡ


ע헡

1 ƷHw\HڙzywѪѪ{zyY(ji)\ָʾ֮һ

2 28lȡĘӱ(yng)Ҝƽ30MЙzy

3 횴_ƷӘĜʴ_ӘӲʴ_ܕ(do)e`ԇY(ji)

4 ʹ΢ҺֹӘӕr(yng)ÿθQ^ȡӱ

5 ڲ^Б(yng)Mⷴ(yng)΢Юa(chn)

6 sϴҺϵߝ}ܕγɽY(ji)δȫܽӰԇY(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽ⣬Ȼϡጻʹ

7 z؏(f)ʹ

ˮԡ偕rՈ(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37档

9ϴ壺

1ϴCϴrϴCļҺP(gun)ҪȱϴҺ^ܳM(yng)΢ϴΔ(sh)(yng)ԓ5(jng)עzҺ^Ƿ

2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝʴ_

3ϴrõˮՈ𷴏(f)ʹ

4ϴCÿʹǰsˮȥxˮϴɃԷֹܵg

10ʹȫԄø˷ϵy(tng)ܕϵy(tng)`(yng)ϵy(tng)(sh)O(sh)m{(dio)ԝMP(gun)|(zh)ƷҪ

11κԇ|Ƥw۾ôˮԓλMДUϴKֹҺᣬиg

12ԇЃ(ni)P(gun)MݼRӱҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎