a(chn)ƷQ
ͨQCyԇУ߱ȝᷨ
bҎ(gu)
ԇ1R1150mlԇ2R2110ml
ԇ1R1250mlԇ2R2210ml
ԇ1R1350mlԇ2R2215ml
ԇ1R1450mlԇ2R2220ml
4x60TR1164ml R281.6ml
8x60TR1324ml R2161.6ml
ԇ1R1140mlԇ2R218ml
A;
ƷѪCȵwzyo\ࡣ
zԭ
zwȝԭӱеCcż(lin)zwϵĿCwخԽYϣp̶ȿɱWxzyӱCcpɱʹöcˣ˘ӱ@õֵ̖A˜ӋCĝȣmg/L
ҪMɳɷ֡
ԇ1100mM Tris
ԇ2uCzҝҺ
ͬԇû
lЧڡ
ԇa(chn)ܹ֮]2-8oCЧڞ12xϵ_ƿԇԷ(wn)4ܡ
a(chn)ڼЧҊ˺
mÃx
a(chn)ƷҪm_ʽȫԄxhÑЃxʹña(chn)Ʒǰ(j)rMC
1HITACHI 7060708071807600̖_ʽȫԄx
2OLYMPUS AU 400AU640AU2700AU5400̖_ʽȫԄx
3Abbott C8000̖_ʽȫԄx
4Roche Modular P800̖_ʽȫԄx
5BECKMAN DXC800̖_ʽȫԄx
6~400800̖_ʽȫԄx
7TRXL-MAX̖ȫԄx
8|֥120̖_ʽȫԄx
9|ܛ400̖_ʽȫԄx
10Bayer ADVIA 1650/2400̖_ʽȫԄx
ӱҪ
1.ѪѪ{EDTAѪ{ӱɼMxѪѪ{
2.2-8惦Ęӱ12(ni)yt-20汣档ӱҜ½yǰ횻Ͼ
z
1. ԇʂ䣺ԇ鼴
2. ԇlL570nmɫ⏽1.0cmض37
|
հ |
УƷ |
ӱ |
R1l |
250 |
250 |
250 |
հףl |
3 |
|
|
УƷl |
|
3 |
|
ӱl |
|
|
3 |
37ԡ5min |
|||
R2l |
50 |
50 |
50 |
37ԡ1minxȡA1 |
|||
37^m(x)ԡ2minxȡA2 |
A=[(A2-A1)УƷܻƷ]-[(A2-A1)հ]
7060ȫԄx(sh)±ʾ
|
37 |
|
cKc |
L/L |
800nm/570nm |
R1/s/R2 |
250l/3l /50l |
MϷʽ |
Spline |
A1yc |
19 |
|
|
A2yc |
25 |
3. Уʼ|(zh)Ƴ
ڌӱMЙzyrʹÌõУƷMж˺ٌõ|(zh)ƷMЙzyzyYS(ni)܌ӱMЙzy
4. ԇYӋ㷽
0.9%}ˮ0ͬԇṩĶƷMжcԝȞXSAYS˜úmǾMϷʽlogitspline˘ӱA˜Ӌֵ
^(q)g
0.5-1.03mg/LhҴ_Ⱥą
zYĽጡ
ӱѪtĝ460mg/dLđt؝ȡ18.2mg/dL֬ȡ500mg/dLSCȡ50mg/dLLӺ450IU/mlyoɔ_
zľԡ
1. ԇľԷ_8.0mg/LӱCȸ8.0mg/Lrʹ}ˮMϡጺٜyyYϡጱ(sh)
2. ԇ2-8oCɃ
3. zԇzy֮ҪxMмrصϴwϢԃxa(chn)
a(chn)Ʒָˡ
1^R1oɫSɫҺwR2ɫҝҺ
2 ݘʾ
3ԇհȣ570nm37&1.0cm⏽0.60AҺ
4`ȣԇyԇ4.0mg/LCӱrȲֵA0.02A
5ԅ^(q)g[08.0]mg/L(ni)
a)Ӌ-Pϵ(sh)r0.990
b) [0.002.00]mg/L^(q)g(ni)ƫ^0.2mg/L[2.018.00]mg/L^(q)g(ni)ƫ^10%
6ܶȣ
6.1؏CV5.0%ӱȞ1.000.10mg/L
6.2g10%ӱȞ1.000.10mg/L
7ʴ_ȣyԇurҎ(gu)ą|(zh)Ycʾֵƫ^15%
8(wn)ԣԇa(chn)ܹ֮]28ɷ(wn)12
ע헡
1. ԇЃHw\ʹá
2. ̖ͬԇû
3. ԇкзBcՈֱӽ|Ƥw۾һ|ôˮ_ϴՈ̷
4. ԇеĜyYHNRo\(j)
5. ԇ͘ӱɸ(j)xIJͬ׃
īI
1.w\ԇRоgָԭtԇУ
2.R҅Ķxʹ_
3. 2006|ϴWġȫRzҎ(gu)̡
4.RWw\ԇУͨügҪ