a(chn)ƷQ
ͨQTᰱD(zhun)øyԇУBm(x)O(jin)y
bҎ(gu)
|
Ҏ(gu)1 |
Ҏ(gu)2 |
Ҏ(gu)3 |
Ҏ(gu)4 |
Ҏ(gu)5 |
Ҏ(gu)6 |
ԇ 1 |
240ml | 460ml | 280ml | 450ml | 440ml | 460ml |
ԇ 2 |
210ml | 230ml | 220ml | 225ml | 220ml |
160ml |
A(y);
t(y)C(gu)wyѪ壨Ѫ{ӱTᰱD(zhun)øĻo\á
ѪеTD(zhun)øVڸKļǸ켡IXͼt@ЩMprTD(zhun)ø@ЩMጳʹѪTD(zhun)øߡ
zԭ
ӱеASTL-Tͦ-ͪᰱD(zhun)QɲL-ȰᣬNADHOÓøMDHᱻ߀ԭL-OᣬNADHNAD+Ķʹ340nm̎Ĺֵ½ͨ^O(jin)y340nmֵ̎½ԜyAST
ҪMɳɷ֡
R1TrisTrisHClNADH0.3g/LLDH MDH1.2KU/L
R2TrisL-Tᣨ200g/L-ͪ
̖ͬԇиMݲԻQ
lЧڡ
ԇa(chn)⃦ܹ28ɷ(wn)12
a(chn)ڼʧЧҊ(bio)
mÃx
a(chn)ƷҪm_ʽȫԄxhÑЃxʹña(chn)Ʒǰ(j)rMC
Abbott Aeroset/Architect c8000
BeckmanCoulterSynchronCX3/CX4/CX5/CX7/LX20
Bayer ADVIA 1200/1650/2400
HITACHI7020/7060/7060C/7070/7080/7150/7170/7170S/7170A/7180/7600
Konelab 30/60i
OLYMPUS AU 400/600/640/2700/5400
Roche Cobas Mira Plus Roche MODULAR P800/D2400
SHIMADZU CL7200/7300/8000
VITALAB Selectra-XL/E
TOSHIBA 30/30FR/40FR/120FR
~ OmilabTM BS-300/400
Sysmex CHEMIX-180
2000
ӱҪ
Ѫ壬ѪASTҜأ20棩Ա48Сr4¿Ա1-25¿Ա1֬ѪSѪѪܕyӡ
z
1.ԇƣԇֱʹ
2. ԇl
|
37 |
|
10l |
L |
340nm |
ԇR1/R2 |
200/50l |
trg |
60 |
yģʽ |
ʷ |
O(jin)yrg |
120 |
(yng) |
½ |
3Уʼ|(zh)Ƴ
@ҪУƷڌӱMЙzyrʹƷԴԵ|(zh)ƷMЙzyzyY(ji)S(ni)܌ӱMЙzy
4ԇY(ji)Ӌ㷽
Kӣ |
TV1000 |
6.22SVP |
ASTU/LA/minK
ASTU/LA/min4180
ʽ TV(yng)weml SVӱweml
6.22=NADH340 nm̎ĺĦϵ(sh) Pɫ⏽cm
KڲͬxϿвͬhҽԼK
^(q)g
<42U/L (37) õąֵֵHhC@һԼąֵ
zY(ji)Ľጡ
Ѫtס4g/LSC0.4g/Lɔ_Y(ji)
zľԡ
(dng)zY(ji)800U/LrҪѪӱϡጺ(f)
a(chn)Ʒָ(bio)
1. ԇ^R1: oɫSɫҺwR2: oɫSɫҺw
2. ݘ(bio)ʾ
3. ԇհȣ1.0 AL340nm⏽1.0cmԇհ׃ʣ0.004A/min
4. `ȣԇyԇ100U/LASTӱr׃ֵA0.012A/min
5. Է0800U/L(ni)Ӌ-(yng)P(gun)ϵ(sh)r0.990ƫܡ15%^ƫ8U/LcӋã
6. yܶȣ؏(f)ԑ(yng)5%g(yng)5%
7. ʴ_ȣyԇurҎ(gu)C|(zh)Y(ji)c(bio)ʾֵƫ(yng)ܡ10%
8. ԇķ(wn)ԣԇa(chn)⃦ܹ2~8ɷ(wn)ʮ헼g(sh)ָ(bio)g⣩ڷ֮(ni)
ע헡
1. ֬S(bio)340nm̎^շ@Щ(bio)иˮƽAST(do)µıM340nmȻSָ߹ֵ˕rƷ(yng)ԓϡጺٜyԇ
2. ԇHw\࣬зNaN3ܿ
īI
1. IFCC methods for the measurement of catalytic enzymes. J Clin Chem Clin Biochem 1986; 24:497-510
2. Gella FJ, et al. Clin Chim Acta 1985; 241-257
3. Young, D.S.et al. Clin Chem 1975; 21:5