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TᰱD(zhun)øyԇ_ϺsʢˎI(y)ɷ޹˾
ԇ
\ \

TᰱD(zhun)øyԇ

TᰱD(zhun)øyԇ

a(chn)ƷQ

ͨQTᰱD(zhun)øyԇУBm(x)O(jin)y

bҎ(gu)

 

Ҏ(gu)1

Ҏ(gu)2

Ҏ(gu)3

Ҏ(gu)4

Ҏ(gu)5

Ҏ(gu)6

ԇ 1

240ml 460ml 280ml 450ml 440ml 460ml

ԇ 2

210ml 230ml 220ml 225ml 220ml

160ml


A(y);

t(y)C(gu)wyѪ壨Ѫ{ӱTᰱD(zhun)øĻo\á

ѪеTD(zhun)øVڸKļǸ켡IX׼ͼt@ЩMprTD(zhun)ø@ЩMጳʹѪTD(zhun)øߡ


ӱеAST߻L-Tͦ-ͪᰱD(zhun)QɲL-ȰᣬNADHOÓøMDHᱻ߀ԭL-OᣬNADHNAD+Ķʹ340nm̎Ĺֵ½ͨ^O(jin)y340nmֵ̎½ԜyAST


ҪMɳɷ֡

R1TrisTrisHClNADH0.3g/LLDH MDH1.2KU/L

R2TrisL-Tᣨ200g/L-ͪ

̖ͬԇиMݲԻQ


lЧڡ

ԇa(chn)⃦ܹ28ɷ(wn)12

a(chn)ڼʧЧҊ(bio)


mÃx

a(chn)ƷҪm_ʽȫԄxhÑЃxʹña(chn)Ʒǰ(j)rMC

Abbott Aeroset/Architect c8000

BeckmanCoulterSynchronCX3/CX4/CX5/CX7/LX20

Bayer ADVIA 1200/1650/2400

HITACHI7020/7060/7060C/7070/7080/7150/7170/7170S/7170A/7180/7600

Konelab 30/60i

OLYMPUS AU 400/600/640/2700/5400

Roche Cobas Mira Plus Roche MODULAR P800/D2400

SHIMADZU CL7200/7300/8000

VITALAB Selectra-XL/E

TOSHIBA 30/30FR/40FR/120FR

~ OmilabTM BS-300/400

Sysmex CHEMIX-180

2000


ӱҪ

Ѫ壬ѪASTҜأ20棩Ա48Сr4¿Ա1-25¿Ա1֬ѪSѪѪܕyӡ


z򞷽

1.ԇƣԇֱʹ

2. ԇl

    

37

10l

    L

340nm

ԇR1/R2

200/50l

trg

60

yģʽ

ʷ

O(jin)yrg

120

(yng)

½

3Уʼ|(zh)Ƴ

@򞲻ҪУƷڌӱMЙzyrʹƷԴԵ|(zh)ƷMЙzyzyY(ji)S(ni)܌ӱMЙzy

 4ԇY(ji)Ӌ㷽

 TV1000

 6.22SVP

ASTU/LA/minK 

ASTU/LA/min4180

ʽ TV(yng)weml SVӱweml

6.22=NADH340 nm̎ĺĦϵ(sh) Pɫ⏽cm

KڲͬxϿвͬhҽԼK


^(q)g

<42U/L (37) õąֵֵHhC@һԼąֵ


zY(ji)Ľጡ

Ѫtס4g/LSC0.4g/Lɔ_Y(ji)


z򞷽ľԡ

(dng)zY(ji)800U/LrҪѪӱϡጺ(f)


a(chn)Ʒָ(bio)

1. ԇ^R1: oɫSɫҺwR2: oɫSɫҺw

2. ݘ(bio)ʾ

3. ԇհȣ1.0 AL340nm⏽1.0cmԇհ׃ʣ0.004A/min

4. `ȣԇyԇ100U/LASTӱr׃ֵA0.012A/min

5. Է0800U/L(ni)Ӌ-(yng)P(gun)ϵ(sh)r0.990ƫܡ15%^ƫ8U/LcӋã 

6. yܶȣ؏(f)ԑ(yng)5%g(yng)5%

7. ʴ_ȣyԇurҎ(gu)C|(zh)Y(ji)c(bio)ʾֵƫ(yng)ܡ10%

8. ԇķ(wn)ԣԇa(chn)⃦ܹ2~8ɷ(wn)ʮ헼g(sh)ָ(bio)g⣩ڷ֮(ni)


ע헡

1. ֬S(bio)340nm̎^շ@Щ(bio)иˮƽAST(do)µıM340nmȻSָ߹ֵ˕rƷ(yng)ԓϡጺٜyԇ

2. ԇHw\࣬зNaN3ܿ


īI

1. IFCC methods for the measurement of catalytic enzymes. J Clin Chem Clin Biochem 1986; 24:497-510

2. Gella FJ, et al. Clin Chim Acta 1985; 241-257

3. Young, D.S.et al. Clin Chem 1975; 21:5