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ᰱD(zhun)øy(c)ԇ_ϺsʢˎI(y)ɷ޹˾
ԇ
\ \

ᰱD(zhun)øy(c)ԇ

ᰱD(zhun)øy(c)ԇ

a(chn)ƷQ
ͨQᰱD(zhun)øy(c)ԇУBm(x)O(jin)y(c)

bҎ(gu)

 

Ҏ(gu)1

Ҏ(gu)2

Ҏ(gu)3

Ҏ(gu)4

Ҏ(gu)5

Ҏ(gu)6

ԇ 1

240ml

460ml

280ml

450ml

440ml

460ml

ԇ 2

210ml

230ml

220ml

225ml

220ml

160ml


A(y);

t(y)C(j)(gu)wy(c)ѪӱбᰱD(zhun)øĻo\

ѪеıᰱD(zhun)øVڸKļǸ켡IXΡ׼(x)ͼt(x)@ЩMpr(sh)ᰱD(zhun)ø@ЩMጳʹѪбᰱD(zhun)ø

z(yn)ԭ

ALTĴ߻İD(zhun)Ƶ-ͪɱͪἰȰᡣͪcNADHLDHĴ߻·(yng)NAD+ NADHڲL(zhng)340nmخշcѪALTĻȣ340nm̎y(c)NADH½Ӌ(j)ALT

ҪMɳɷ֡

R1

ԭQ

Tris

2.5g/L

Tris.HCl

18g/L

Óø

4KU/L

NADH

0.3g/L







R2

ԭQ

Tris

4.1g/L

L-

196g/L

-ͪ

12.15g/L






̖ͬ(ho)ԇиMݲԻQʹ


(ch)lЧڡ

ԇa(chn)⃦ܹ֮(ch)2~8ɷ(wn)12(g)

a(chn)ڼʧЧҊ(bio)


mÃx

a(chn)ƷҪm_ʽȫԄ(dng)xhÑЃxʹña(chn)Ʒǰ(j)(sh)(yn)rM(jn)(yn)C

Abbott Aeroset/Architect c8000

BeckmanCoulterSynchronCX3/CX4/CX5/CX7/LX20

Bayer ADVIA 1200/1650/2400

HITACHI7020/7060/7060C/7070/7080/7150/7170/7170S/7170A/7180/7600

Konelab 30/60i

OLYMPUS AU 400/600/640/2700/5400

Roche Cobas Mira Plus  Roche  MODULAR P800/D2400

SHIMADZU CL7200/7300/8000

VITALAB Selectra-XL/E

TOSHIBA 30/30FR/40FR/120FR

~ OmilabTM BS-300/400

Sysmex CHEMIX-180

2000

ӱҪ
ѪѪALTҜأ20棩Ա48Сr(sh)2-8¿Ա1-25¿Ա1(g)¡(yn)֬ѪSѪѪ壬ܕ(hu)y(c)

z(yn)

1. ԇƣԇҺwֱʹ

2. ԇ(yn)l

    

37

10l

    L(zhng)

340nm

ԇR1/R2

200/50l

tr(sh)g

60

y(c)ģʽ

ʷ

(yng)r(sh)g

120

(yng)

½

3У(zhn)|(zh)Ƴ

@(g)(sh)(yn)ҪУ(zhn)Ʒڌ(du)ӱM(jn)Йzy(c)r(sh)ʹƷԴԵ|(zh)ƷM(jn)Йzy(c)zy(c)Y(ji)S(ni)܌(du)ӱM(jn)Йzy(c)

4ԇ(yn)Y(ji)Ӌ(j)㷽

 Kӣ

 TV1000

 6.22SVP


ALTU/LA/minK    

ALTU/LA/min4180

ʽ TV(yng)weml SVӱweml

6.22=NADH340 nm̎ĺĦϵ(sh) Pɫ⏽cm

KڲͬxϿвͬh(sh)(yn)ҽԼK

^(q)g

< 41 U/L(37)

õąֵֵHh(sh)(yn)(yn)C@һԼąֵ


z(yn)Y(ji)Ľጡ

Ѫtס4g/LSC0.4g/Lɔ_Y(ji)

z(yn)ľԡ
(dng)z(yn)Y(ji)800U/Lr(sh)ҪѪӱϡጺ(f)


a(chn)Ʒָ(bio)

1. ԇ^R1: oɫSɫҺwR2: oɫSɫҺw

2. ݘ(bio)ʾ

3. ԇհȣ1.0 AL(zhng)340nm⏽1.0cmԇհ׃ʣ0.004A/min

4. `ȣԇy(c)ԇ100U/LALTӱr(sh)׃ֵA0.012A/min

5. Է0800U/L(ni)Ӌ(j)-(yng)P(gun)ϵ(sh)r0.990ƫܡ15%^(du)ƫ8U/Lc(din)Ӌ(j)ã 

6. y(c)ܶȣ؏(f)ԑ(yng)5%g(yng)5%

7. (zhn)_ȣy(c)ԇu(png)r(ji)Ҏ(gu)C|(zh)Y(ji)c(bio)ʾֵ(du)ƫ(yng)ܡ10%

8 ԇķ(wn)ԣԇa(chn)⃦ܹ(ch)2~8ɷ(wn)ʮ(g)(xing)g(sh)ָ(bio)g⣩ڷ֮(ni)


ע(xing)

1. ֬S(bio)340nm̎^(qing)շ壬@Щ(bio)иˮƽALT(hu)(do)µıM340nmȻSָ߹ֵ˕r(sh)Ʒ(yng)ԓϡጺٜy(c)ԇ

2. tr(sh)g(ni)ӱи߱ͪẬ(hu)NADHĶ͜y(c)ԇľ

3. ԇЃHw\ʹá

4. ԇкзͷ(wn)ܴһĴ̼ûՈ(qng)ֱӽ|Ƥw۾һ|ôˮ_ϴՈ(qng)̷


īI(xin)


1 1 IFCC methods for the measurement of catalytic concentration of enzymes. J Clin Chem Clin Biochem 1986; 24: 481-495

2. Gella FJ, et al. Clin Chim Acta 1985; 153:241-247