a(chn)ƷQ
ͨQᰱD(zhun)øy(c)ԇУBm(x)O(jin)y(c)
bҎ(gu)
|
Ҏ(gu)1 |
Ҏ(gu)2 |
Ҏ(gu)3 |
Ҏ(gu)4 |
Ҏ(gu)5 |
Ҏ(gu)6 |
ԇ 1 |
240ml |
460ml |
280ml |
450ml |
440ml |
460ml |
ԇ 2 |
210ml |
230ml |
220ml |
225ml |
220ml |
160ml |
R1
ԭQ |
|
Tris |
2.5g/L |
Tris.HCl |
18g/L |
Óø |
4KU/L |
NADH |
0.3g/L |
R2
ԭQ |
|
Tris |
4.1g/L |
L- |
196g/L |
-ͪ |
12.15g/L |
̖ͬ(ho)ԇиMݲԻQʹ
(ch)lЧڡ
ԇa(chn)⃦ܹ֮(ch)2~8ɷ(wn)12(g)
a(chn)ڼʧЧҊ(bio)
mÃx
a(chn)ƷҪm_ʽȫԄ(dng)xhÑЃxʹña(chn)Ʒǰ(j)(sh)(yn)rM(jn)(yn)C
Abbott Aeroset/Architect c8000
BeckmanCoulterSynchronCX3/CX4/CX5/CX7/LX20
Bayer ADVIA 1200/1650/2400
HITACHI7020/7060/7060C/7070/7080/7150/7170/7170S/7170A/7180/7600
Konelab 30/60i
OLYMPUS AU 400/600/640/2700/5400
Roche Cobas Mira Plus Roche MODULAR P800/D2400
SHIMADZU CL7200/7300/8000
VITALAB Selectra-XL/E
TOSHIBA 30/30FR/40FR/120FR
~ OmilabTM BS-300/400
Sysmex CHEMIX-180
2000
ӱҪ
ѪѪALTҜأ20棩Ա48Сr(sh)2-8¿Ա1-25¿Ա1(g)¡(yn)֬ѪSѪѪ壬ܕ(hu)y(c)
z(yn)
1. ԇƣԇҺwֱʹ
2. ԇ(yn)l
|
37 |
|
10l |
L(zhng) |
340nm |
ԇR1/R2 |
200/50l |
tr(sh)g |
60 |
y(c)ģʽ |
ʷ |
(yng)r(sh)g |
120 |
(yng) |
½ |
3У(zhn)|(zh)Ƴ
@(g)(sh)(yn)ҪУ(zhn)Ʒڌ(du)ӱM(jn)Йzy(c)r(sh)ʹƷԴԵ|(zh)ƷM(jn)Йzy(c)zy(c)Y(ji)S(ni)܌(du)ӱM(jn)Йzy(c)
4ԇ(yn)Y(ji)Ӌ(j)㷽
Kӣ |
TV1000 |
6.22SVP |
ALTU/LA/minK
ALTU/LA/min4180
ʽ TV(yng)weml SVӱweml
6.22=NADH340 nm̎ĺĦϵ(sh) Pɫ⏽cm
KڲͬxϿвͬh(sh)(yn)ҽԼK
^(q)g
< 41 U/L(37)
õąֵֵHh(sh)(yn)(yn)C@һԼąֵ
z(yn)Y(ji)Ľጡ
Ѫtס4g/LSC0.4g/Lɔ_Y(ji)
z(yn)ľԡ
(dng)z(yn)Y(ji)800U/Lr(sh)ҪѪӱϡጺ(f)
a(chn)Ʒָ(bio)
1. ԇ^R1: oɫSɫҺwR2: oɫSɫҺw
2. ݘ(bio)ʾ
3. ԇհȣ1.0 AL(zhng)340nm⏽1.0cmԇհ׃ʣ0.004A/min
4. `ȣԇy(c)ԇ100U/LALTӱr(sh)׃ֵA0.012A/min
5. Է0800U/L(ni)Ӌ(j)-(yng)P(gun)ϵ(sh)r0.990ƫܡ15%^(du)ƫ8U/Lc(din)Ӌ(j)ã
6. y(c)ܶȣ؏(f)ԑ(yng)5%g(yng)5%
7. (zhn)_ȣy(c)ԇu(png)r(ji)Ҏ(gu)C|(zh)Y(ji)c(bio)ʾֵ(du)ƫ(yng)ܡ10%
8 ԇķ(wn)ԣԇa(chn)⃦ܹ(ch)2~8ɷ(wn)ʮ(g)(xing)g(sh)ָ(bio)g⣩ڷ֮(ni)
ע(xing)
1. ֬S(bio)340nm̎^(qing)շ壬@Щ(bio)иˮƽALT(hu)(do)µıM340nmȻSָ߹ֵ˕r(sh)Ʒ(yng)ԓϡጺٜy(c)ԇ
2. tr(sh)g(ni)ӱи߱ͪẬ(hu)NADHĶ͜y(c)ԇľ
3. ԇЃHw\ʹá
4. ԇкзͷ(wn)ܴһĴ̼ûՈ(qng)ֱӽ|Ƥw۾һ|ôˮ_ϴՈ(qng)̷
īI(xin)
1 1 IFCC methods for the measurement of catalytic concentration of enzymes. J Clin Chem Clin Biochem 1986; 24: 481-495
2. Gella FJ, et al. Clin Chim Acta 1985; 153:241-247