a(chn)ƷQ
ͨQײIgGwzyԇУø(lin)߷
bҎ(gu)
48 ˷/У96 ˷/
A(y);
a(chn)ƷwⶨԙzyѪײIgGw
Y(ji)HRR\εΨһ(j)ײȾo\ࡣ
ϢB
ײHEVСs2734nmĆι期oĤRNAMȫLs7.5kb3_xaܣORFORF1ac(f)P(gun)ĹԵORF2cORF3ƜyaĽY(ji)(gu)ORF2 C˾aҪY(ji)(gu)ҪĿԭλcо߂ͨ^ϳĿԭؽMԭоl(f)F(xin)˶ԭλоORF2ؽMԭcϳĿԭELISAzyHEVwR\
(Q) ײHepatitis E Virus, HEVȾRIJʸڼףҪڰl(f)չЇ,DŮȾHEV䲡ɸ_(d)20%,3µDŮ߿_(d)56%YоһNȾDZo(j)80ĩl(f)F(xin)µIJԸ֮һȫУVҊځc^(q)҇DzԸ߰l(f)^(q)(j)y(tng)ӋڼԲԸװl(f)ʞ3.420.5ƽ8.6
GENELABS®ײIgMIgG ELISAzyԇΨһ@ÇˎƷM(jn)עCײzyM(jn)ԇ GENELABSHEV IgMIgGԇвø(lin)ײMضйԵĻƬքezywѪѪ{пHEV IgMIgG@N(bio)־ؽMƬγɹؑ(yng)ڲͬĵطЅ^(q)ļȾ\ѪW(xu)HEV IgMIgGzy\ײȾҪָ(bio)֮һ
zԭ
ƷɻؽM_(d)ײHEVԭװӴyѪ, (dng)yƷдײwtγɿԭwخY(ji)ټ뿹IgGøY(ji)c䷴(yng)(jng)ø@ɫø(bio)xϜy(j)ODֵжПoײ IgGwĴ
ҪMɳ
1ײԭװ |
96ס1K |
48ס1K |
2øY(ji) |
10mL1ƿ |
5mL1ƿ |
3Ԍ |
1mL1ƿ |
1mL1ƿ |
4Ԍ |
1mL1ƿ |
1mL1ƿ |
5ϴҺǰ120ϡጣ |
50mL1ƿ |
50mL1ƿ |
6@ɫ A |
6mL1ƿ |
3mL1ƿ |
7@ɫ |
6mL1ƿ |
3mL1ƿ |
8KֹҺ |
6mL1ƿ |
3mL1ƿ |
9ƷϡҺ |
10mL1ƿ |
5mL1ƿ |
(10) zƬ |
3 |
3 |
MݽMɣ1ײԭװ壺ûؽM_(d)ײ14͵ORF2ORF3λԭϰϩװ2øY(ji)50%СţѪ壬15%ɽѪ0.1%0.01M}_Һ(pH7.4)3ԌգײIgGwzyѪ4ԌգײIgGwzyѪ5ϴҺ}_Һ-0.5206@ɫ A8.00g ,^0.70g; 7@ɫB2.10gҶ0.38g3,3',5,5'-ļ(lin) 0.40g8KֹҺ2M H2SO49ƷϡҺ0.24%u飨Tris_Һ1.78%NaCl0.5%ҵc0.2%ţѪ0.1%-200.1%ͨX-1005%ɽѪ0.1%
lЧ
ԇБ(yng)2~8бܹⱣ棬Ч12δİװl(yng)Էܷcʣԇ2~8汣һ܃(ni)ꡣ\ݔ5첻Ӱ䷀(wn)
a(chn)ڼЧҊ(bio)
mÃx
ø(bio)xzL450nmL630nm
ӱҪ
1ӱɼҪ(zhn)䣬IJѪg(sh)ռѪҺȫѪƷȷ37̎2СrٌѪƷxģ3000D(zhun)/x6ʹѪ岻OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr
2ӱʹѪػEDTAȿӰ푌Y(ji)
3ӱкïBcӰ푌Y(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY(ji)
4rĘӱ2~8±һڣrӱLڃ-20ⷴ(f)ڡ
z
1(zhn)䣺حh(hun)ȡԇУҜƽ30ͬrsϴҺ1:20ϡ
2ӴyӱÿΌO(sh)ԌԌոɿքeԌѪ100LO(sh)һհӘƷϡҺ100L100LƷϡҺټӴyѪ10Lֻ3730ȥ׃(ni)ӱ۸ɡ
3ϴ壺1ֹϴ壺ȥװl׃(ni)ҺwˮĸɣϴҺעMÿo5~10ȥ׃(ni)ϴҺĸ˷(f)5ĸ
2ϴCϴ壺xϴ5εijϴϴҺ(yng)עMÿ_ÿoˮĸɡ
4øY(ji)ÿ100LհտײֻNճ3720
5ϴ壺ͬ3
6@ɫȼ@ɫAҺ50L ټ@ɫBҺ50Lֻ37 ܹ10
7Kֹ(yng)ÿӽKֹҺ50L
8yø(bio)xx(sh)xÆβL450nm(ԿհУ)450/630nmxȡODֵx(sh)ڽKֹ10犃(ni)ɡ
Дֵ
Cutoff ֵӋ㣺COV=ԌƽODֵ0.12
(bio)ODֵCOV(bio)ODֵ
עԌODֵ0.050.05Ӌ㣬0.05HODֵӋ
zY(ji)Ľ
1ԽY(ji)ӱвײIgGwӱеײIgGwԇеęzy
2ԽY(ji)ӱкײIgGwخ(yng)ء
3ԽY(ji)ĘӱҪM(jn)؏(f)ֻн(jng)^؏(f)؏(f)ԞԽY(ji)ĘӱJ(rn)ײIgGw؏(f)Y(ji)ԵĘӱ(yng)(jng)^m(dng)?sh)ֶM(jn)д_J(rn)
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)F(xin)ԽY(ji)܌һg(sh)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥصס
4ԇڼԡl(f)Ŀ93.8%خ94.4%
ԇľ
ƷHڙzyѪײIgGwwzyRo\
a(chn)Ʒָ(bio)
1.ԇ^Һwo
2.10ײwIgGԅƷzy(yng)ȫz
3.10ײwIgGԅƷzy(yng)ȫz
4.4ײwIgG`ȅƷzyS1S2S3 (yng)ԣS4
5.1ײwIgGԅƷzyCV%(yng)15n=10
6.(wn)ԌԇM376zY(ji)(yng)_(d)(bio)(zhn)
7.bݘ(bio)ʾb
8.֬ѪSȸɔ_ӌzyY(ji)Ӱ푣Ȟ2.1mmol/L(ֵ0.22~1.65mmol/L)ѪtםȞ210g/L(ֵ110~170g/L), đ̴Ȟ8.5 mmol/L(ֵ:2.6~6.5mmol/L)Ӱ푱ƷzyY(ji)
9.cԭwĽ淴(yng)о:HAV16HBV23HCV24HIV26TORCH 18M(jn)н淴(yng)ԇY(ji)Ӱ푱ƷęzyY(ji)
10.RоϢ飺оԇc(zhn)ײIgGwzyԇR(bio)M(jn)Ќșzy^خԵȷĵЧ
ĿӱĴ_(j)(j)w\ԇעԹkҎ(gu)оa(chn)Ʒa(chn)Ʒ(j)w\ԇRоg(sh)ָ(do)ԭtRоӱ(sh)1314оY(ji)93.8%;خ94.4%;ӱ94.1%
ע
1 ƷHw\࣬HڙzywѪzyY(ji)HRR\εΨһ(j)
2 2~8lȡĘӱ(yng)Ҝƽ30M(jn)Йzy
3 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(do)e`ČY(ji)
4 ʹҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ
5 õƿμӕrՈƿҺwuȥ1~2κֱٵصμҪ⌢MеҺw|Rװ߅ϣeøY(ji)
6 ڲ^Б(yng)Mⷴ(yng)Юa(chn)
7 sϴҺϵߝ}ܕγɽY(ji)δȫܽӰ푌Y(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽȻϡጻʹ
8 z؏(f)ʹ
9 ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37
10ϴ壺1ϴCϴrϴCļҺP(gun)ҪȱϴҺ^ܳM(yng)ϴΔ(sh)(yng)ԓ5(jng)עzҺ^Ƿ
2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝ(zhn)_
3ϴrõˮՈ(f)ʹá
4ϴCÿʹǰsˮȥxˮϴɃԷֹܵg
11ʹȫԄø(bio)ϵy(tng)ܕϵy(tng)`(yng)ϵy(tng)(sh)O(sh)m(dng){(dio)ԝMP(gun)|(zh)ƷҪ
12κԇ|Ƥw۾ôˮԓλM(jn)ДU(ku)ϴKֹҺиgԡ
13ԇЃ(ni)P(gun)MݼRӱҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎
īI(xin)
1.R҅Ķxʹ_
2. 2006|ϴW(xu)ġȫRzҎ(gu)̡
3.RW(xu)w\ԇУͨüg(sh)Ҫ
t(y)еa(chn)I(y)SC̖
t(y)еעC̖/a(chn)Ʒg(sh)Ҫ̖
f˜(zhn)ڼڡ