aƷQ
ͨQײĿwzyԇУø߷
bҎ(gu)
48˷/96˷/С576˷/
A;
aƷwⶨԙzyѪѪ{ײĿw
ײHBVȾR\ָ֮һ
zԭ
ײĿwzyԇвøƷzyҸκĿw-HBcûؽMҸκĿԭHBcAglHRPӛĿ-HBcøӛļTMB^y˱ͬr뿹-HBc-HRPcԭγɸYϣ˱п-HBcߕrt-HBc-HRPcҸκĿԭHBcAgYTMBr@ɫ֮t@ɫڌYroɫɫ׃ʾҸκĿw-HBc@ɫtʾoҸκĿw-HBc
ҪMɳɷ֡
ԇнM |
48˷/ |
96˷/ |
576˷/ |
1.A |
48/K1K |
96/K1K |
96/K6K |
2.øY |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
3.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
4.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
5.sϴҺ |
30mL1ƿ |
30mL1ƿ |
100mL2ƿ |
6.@ɫA |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
7.@ɫB |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
8.KֹҺ2M H2SO4 |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
9.z |
1Ƭ |
1Ƭ |
6Ƭ |
10.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬԇԇнM12 ɻá
MݽMɣ
øYNa2HPO412H2O 6.7g/LNaH2PO42H2O 0.16g/LKCl 0.2g/LNaCl 8g/LwAøo0.6g/LСţѪ 50mL/LҺwAҺw7mL/Lָʾ1 1.25mL/LHBcAb-HRPm
ԌգNa2HPO412H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/LNaN3 60g/LҶ 200mL/LСţѪ 200mL/Lָʾ1 1.25mL/LѪm
ԌգNa2HPO412H2O 6.7g/LNaH2PO42H2O 0.16g/L KCl 0.2g/L NaCl 8g/LҺwAҺw 5mL/Lָʾ2 0.55mL/LѪ 200mL/L
sϴҺNa2HPO412H2O 58g/L NaCl 160g/LKCl 4g/LKH2PO4 4g/LTween-20 10 mL/L
@ɫA 8g/LԇA 0.004g/LԇB 2.4mL/L^ 0.7g/L
@ɫB 2.1g/LҶc 0.38g/LTMB 0.4g/L
KֹҺ 2M H2SO4
ҪδṩƷ
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌr
7. ϴCϴƿC
8. ʎ
lЧڡ
ԇБ28бܹⱣЧ12
aڼЧҊ˺
mÃx
ø˃x zyL450nmL630nm
ӱҪ
1. ӱɼҪʂҪܙz߽ʳIJѪgռѪҺȫѪƷȷ37̎2СrٌѪƷxģ3000D/x6ʹѪ壨{OٺѪ鿹ѪƷѪƷxĺٷҜ2Сr
2. ӱʹѪѪ{ᡢػEDTAȿӰ푌Y
3. ӱкïBcӰ푌ĽY߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY
4. rĘӱ28ol¿ɱһrӱLA-20ⷴ̓
z
1. ʂ䣺حh(hun)ȡԇУҜƽ30ͬrsϴҺ120ϡጡ
2. Ӵy˱y˱ÿ0.05yӱ1:30ϡጣOԌ2Ԍ2ף0.05հ1
3. øYÿ0.05հտײֻNz3730
4. ϴ壺1ֹϴ壺ȥl׃ҺwˮĸϴҺעMÿo510ȥ׃ϴҺĸ˷5ĸ
2ϴCϴ壺xϴ5εijϴ壬ϴҺעMÿ_ÿoˮĸɡ
5. @ɫ ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ15
6. Kֹ ÿKֹҺ0.05
7.yø˃xxxβL450 nmԿհУ㣩pL450/630 nmxȡODֵxڽKֹ10犃
Дֵ
Cutoff ֵӋ㣺 ԭѪCOV = ԌƽODֵ0.3
1:30ϡѪCOV = ԌƽODֵ0.5
˱ODֵCOV˱ODֵCOVԡ
zYĽጡ
1. ԽYӱвҸκĿw-HBcӱеҸκĿw-HBcԇеęzy
2. ԽYӱкҸκĿw-HBcخ
3. ԽYĘӱҪM؏ֻ͌н^؏ͻ؏͌ԞԽYĘӱJҸκĿw-HBc؏ͽYԵĘӱ^mֶMд_J
ԽYĘӱ͙zԽYJԘӱ؏ͲԽY܌һg}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥصס
4. Ѫzy͵ζҸκĿw-HBcֻȾвWxֻЙzyߵζȿw1:30Bm(x)zywζuߕrҸκĿw-HBcſȾָ
zľԡ
иԵߌϵyڝڵķخԷ؏͵ԽYһmķMд_J
aƷָˡ
1 ԇ^Һwo
2 ԅƷʣ15ԅƷzyYóF
3 ԅƷʣ15ԅƷSF1ݼ
4 ͙z͙zƷy1IU/mL
5 ԣþԅƷyCV20n=10
6 (wn)ԇԇM376죬zY_˜15
7 bݘʾb
ע헡
1. ƷHw\HޙzywѪѪ{zyY\ָ֮һ
2. 28lȡĘӱҜƽ30MЙzy
3. 횴_ƷӘĜʴ_ӘӲʴ_ܕe`ČY
4. ʹҺֹӘӕrÿΑԓQ^ȡӱ
5. õƿμӕrՈJҺwuȥ12κֱٵصμҪ⌢MеҺw|R߅eøY
6. ڲ^БMⷴЮaݡ
7. sϴҺϵߝ}ܕγɽYδȫܽӰ푌YͬrܕסԄӼӘϴCܵFYՈ37ܽȻϡጻʹ
8. z؏ʹ
9. ˮԡ偷rՈˮ1/3ײԾW֧֧ˮؿ37
10. ϴ壺1ϴCϴrϴCļҺPҪȱϴҺ^ܳMϴΔ5עzҺ^Ƿ
2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|c@ɫl(f)Ӱ푙zyYĜʴ_
3ϴrõˮՈʹ
4ϴCÿʹǰsˮȥxˮϴɃԷֹ·g
11. κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиgԡ
12. ԇЃPMݼRӱҕНڂȾՈPČҹҎ(gu)(zh)к̎