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͸ײewzy(c)ԇУø“(lin)߷_ϺsʢˎI(y)ɷ޹˾
ԇ
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͸ײewzy(c)ԇУø“(lin)߷

͸ײewzy(c)ԇУø“(lin)߷

a(chn)ƷQ(chng)

ͨQ(chng)͸ײeԭzy(c)ԇУø“(lin)߷


bҎ(gu)

48˷/96˷/С576˷/С


A(y);

ԇmwⶨԙzy(c)ѪѪ{͸ײ

͸ײHBVȾR\ָ(bio)֮һ


z(yn)ԭ

͸ײeԭzy(c)ԇвpwAķzy(c)ҸeԭHBeAgÆοҸeԭ-HBelHRP(bio)ӛĿ-HBeø(bio)ӛļ׻“(lin)TMB^(gu)(dng)(bio)дҸeԭHBeAgr(sh)ԓҸeԭHBeAgc-HBeY(ji)ϲc-HBe-HRPY(ji)γɿ-HBe- HBeAg--HBe-HRP(f)TMBa(chn)@ɫ(yng)֮to(w)@ɫ(yng)ڌ(sh)(yn)Y(ji)r(sh)ɫ׃ģʾҸeԭHBeAgo(w)ɫɫ׃΢tʾҸeԭHBeAg


ҪMɳɷ֡

ԇнM

48˷/

96˷/

576˷/

1.A(y)(yng)

48/K1K

96/K1K

96/K6K

2.øY(ji)

3mL1ƿ

6mL1ƿ

40mL1ƿ

3.(yng)Ԍ(du)

0.5mL1ƿ

0.5mL1ƿ

2mL1ƿ

4.Ԍ(du)

0.5mL1ƿ

0.5mL1ƿ

2mL1ƿ

5.sϴҺ

30mL1ƿ

30mL1ƿ

100mL2ƿ

6.@ɫA

3mL1ƿ

6mL1ƿ

40mL1ƿ

7.@ɫB  

3mL1ƿ

6mL1ƿ

40mL1ƿ

8.KֹҺ2M H2SO4

3mL1ƿ

6mL1ƿ

40mL1ƿ

9.z

10.Է

ע⣺̖ͬ(ho)ԇԇнM12 ɻ

MݽMɣ

øY(ji)Na2HPO412H2O 2.9g/LKH2PO4 0.2g/LKCl 0.2g/LNaCl 8g/Lҵ 1g/LСţѪ 400mL/LҺwAҺw5mL/L 50mL/L ָʾ1  1.25mL/LTween-20  0.5mL/L HBeAb-HRP m

(yng)Ԍ(du)գNa2HPO412H2O  2.9g/LNaCl  8g/LKCl  0.2g/LKH2PO4  0.2g/L  2g/LҶ 200mL/LСţѪ  200mL/Lָʾ1  1.25mL/L ؽMҸeԭHBeAgm

Ԍ(du)գ Na2HPO412H2O 2.9g/L NaCl  8g/LKCl  0.2g/LKH2PO4  0.2g/LNaN3  60g/Lָʾ2  0.555mL/LѪ  300mL/L

sϴҺ Na2HPO412H2O  58g/L NaCl  160g/LKCl  4g/LKH2PO4  4g/LTween-20  10mL/L

A 8g/LԇA 0.004g/LԇB 2.4mL/L^(gu) 0.7g/L

B 2.1g/LҶc 0.38g/LTMB 0.4g/L

KֹҺ 2M H2SO4 

ҪδṩƷ

1. rsˮȥxˮ

2. һ

3. _΢Һ͝^

4. Ƀˮ

5. 37ˮԡ

6. Ӌ(j)r(sh)

7. ϴC(j)ϴƿC(j)

8. ʎ


(ch)lЧڡ

  ԇБ(yng)28бܹⱣ棬Ч12(g)

a(chn)ڼЧҊ(jin)(bio)


mÃx

ø(bio)x zy(c)L(zhng)450nmL(zhng)630nm


ӱҪ

1. ӱɼҪ(zhn)Ҫܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2Сr(sh)ٌѪƷx3000D(zhun)/x6ϣʹѪ壨{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr(sh)

2. ӱʹѪѪ{ػEDTAȿ(hu)Ӱ푌(sh)(yn)Y(ji)

3. ӱкïBc(hu)Ӱ푌(sh)(yn)ĽY(ji)߶ѪĘӱ](mi)ȫտsѪӱ΢ȾĘӱܕ(hu)e(cu)`ĽY(ji)

4. rĘӱ28o(w)l¿ɱһrӱL(zhng)A-20ⷴ(f)ڡ


z(yn)

1. (sh)(yn)(zhn)䣺حh(hun)ȡԇҜƽ30ͬr(sh)sϴҺ120ϡ

2. Ӵy(c)(bio)y(c)(bio)ÿ0.05O(sh)(yng)Ԍ(du)2Ԍ(du)2ף0.05հ׌(du)1

3. øY(ji)ÿ0.05հ׌(du)տײֻNz3730

4. ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעM(mn)ÿףo510ȥ׃(ni)ϴҺĸɣ˷(f)5ĸ

2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עM(mn)ÿף_ÿo(w)ˮĸɡ

5.  ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ15

6. Kֹ(yng) ÿ׼KֹҺ0.05

7. y(c)ø(bio)xx(sh)xβL(zhng)450 nmԿհ׿У㣩pL(zhng)450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)


(yng)Дֵ

Cutoff ֵӋ(j)㣺 COV = Ԍ(du)ƽODֵ2.1

(bio)ODֵCOV(yng)(bio)ODֵCOV

עԌ(du)ODֵ0.050.05Ӌ(j)㣬0.05(sh)HODֵӋ(j)


z(yn)Y(ji)Ľጡ

1. ԽY(ji)ӱвҸeԭHBeAgӱеҸeԭHBeAgԇеęzy(c)

2. (yng)ԽY(ji)ӱкҸeԭHBeAgخ(yng)

3. (yng)ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)ֻн(jng)^(gu)؏(f)؏(f)(sh)(yn)Ԟ(yng)ԽY(ji)ĘӱJ(rn)ҸeԭHBeAg(yng)ԡ؏(f)Y(ji)(yng)ԵĘӱ(yng)(jng)^(gu)m(dng)?sh)ֶM(jn)д_J(rn)

(yng)ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)(yng)ԽY(ji)܌һ(g)(g)g(sh)(wn)}£

1 ڃxӘɵĽȾ

2 @ɫxȾ

3 ԇ©ɵĽȾ

4 ֵϴȥص


z(yn)ľԡ

   иԵߌ(sh)(yn)ϵy(tng)ڝڵķخԷ(yng)؏(f)(yng)ԽY(ji)(yng)һ(g)m(dng)?sh)ķM(jn)д_J(rn)

a(chn)Ʒָ(bio)

1 ԇ^͸Һwo(w)

2 ԅƷʣ15ԅƷzy(c)Y(ji)óF(xin)(yng)

3 (yng)ԅƷʣ10(yng)ԅƷzy(c)Բö1ݡ

4͙z͙zƷy(c)(yng)1IU/mL

5 ԣþԅƷy(c)CV(yng)15n=10

6 (wn)ԇ(yn)ԇM376zY(ji)(yng)_(d)15Ҫ

7 bݘ(bio)ʾb


ע(xing)

1. ƷHw\Hޙzy(c)wѪѪ{zy(c)Y(ji)\ָ(bio)֮һ

2. 28lȡĘӱ(yng)Ҝƽ30M(jn)Йzy(c)

3. 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(hu)(do)e(cu)`Č(sh)(yn)Y(ji)

4. ʹ΢ҺֹӘӕr(sh)ÿΑ(yng)ԓQ^ȡӱ

5. õƿμӕr(sh)Ո(qng)JҺwu򣬲ȥ12κֱٵصμҪ⌢MеҺw|?yn)R΢߅؄eøY(ji)

6. ڲ^(gu)Б(yng)Mⷴ(yng)΢Юa(chn)ݡ

7. sϴҺϵߝ}ܕ(hu)γɽY(ji)δȫܽ⣬(hu)Ӱ푌(sh)(yn)Y(ji)ͬr(sh)ܕ(hu)סԄ(dng)ӘϴC(j)ܵF(xin)Y(ji)Ո(qng)37ܽ⣬Ȼϡጻʹ

8. z؏(f)ʹ

9. ˮԡ偷(yng)r(sh)Ո(qng)(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37档

10. ϴ壺1ϴC(j)ϴr(sh)ϴC(j)ļҺP(gun)ҪȱϴҺ^(gu)ܳM(mn)(yng)΢УϴΔ(sh)(yng)5(jng)עzҺ^Ƿ

2ֹϴr(sh)Ո(qng)ʹÎмˮİԷԴ^(gu)ø(li)߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zy(c)Y(ji)Ĝ(zhn)_

3ϴr(sh)õˮՈ(qng)𷴏(f)ʹ

4ϴC(j)ÿʹǰsˮȥxˮϴɃԷֹ·g

11. κԇ|Ƥw۾ôˮ(du)ԓλM(jn)ДU(ku)ϴKֹҺиg

12. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈ(qng)P(gun)Č(sh)(yn)ҹҎ(gu)(li)(zh)к̎