a(chn)ƷQ(chng)
ͨQ(chng)ײeԭzy(c)ԇУø(lin)߷
bҎ(gu)
48˷/96˷/С576˷/С
A(y);
ԇmwⶨԙzy(c)ѪѪ{ײeԭ
ײHBVȾR\ָ(bio)֮һ
z(yn)ԭ
ײeԭzy(c)ԇвpwAķzy(c)ҸeԭHBeAgÆοҸeԭ-HBelHRP(bio)ӛĿ-HBeø(bio)ӛļ(lin)TMB^(gu)(dng)(bio)дҸeԭHBeAgr(sh)ԓҸeԭHBeAgc-HBeY(ji)ϲc-HBe-HRPY(ji)γɿ-HBe- HBeAg--HBe-HRP(f)TMBa(chn)@ɫ(yng)֮to(w)@ɫ(yng)ڌ(sh)(yn)Y(ji)r(sh)ɫ׃ģʾҸeԭHBeAgo(w)ɫɫ׃tʾҸeԭHBeAg
ҪMɳɷ֡
ԇнM |
48˷/ |
96˷/ |
576˷/ |
1.A(y)(yng) |
48/K1K |
96/K1K |
96/K6K |
2.øY(ji) |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
3.(yng)Ԍ(du) |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
4.Ԍ(du) |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
5.sϴҺ |
30mL1ƿ |
30mL1ƿ |
100mL2ƿ |
6.@ɫA |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
7.@ɫB |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
8.KֹҺ2M H2SO4 |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
9.z |
1Ƭ |
1Ƭ |
6Ƭ |
10.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬ(ho)ԇԇнM12 ɻ
MݽMɣ
øY(ji)Na2HPO412H2O 2.9g/LKH2PO4 0.2g/LKCl 0.2g/LNaCl 8g/Lҵ 1g/LСţѪ 400mL/LҺwAҺw5mL/L 50mL/L ָʾ1 1.25mL/LTween-20 0.5mL/L HBeAb-HRP m
(yng)Ԍ(du)գNa2HPO412H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/L 2g/LҶ 200mL/LСţѪ 200mL/Lָʾ1 1.25mL/L ؽMҸeԭHBeAgm
Ԍ(du)գ Na2HPO412H2O 2.9g/L NaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/LNaN3 60g/Lָʾ2 0.555mL/LѪ 300mL/L
sϴҺ Na2HPO412H2O 58g/L NaCl 160g/LKCl 4g/LKH2PO4 4g/LTween-20 10mL/L
@ɫA 8g/LԇA 0.004g/LԇB 2.4mL/L^(gu) 0.7g/L
@ɫB 2.1g/LҶc 0.38g/LTMB 0.4g/L
KֹҺ 2M H2SO4
ҪδṩƷ
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌ(j)r(sh)
7. ϴC(j)ϴƿC(j)
8. ʎ
(ch)lЧڡ
ԇБ(yng)28бܹⱣ棬Ч12(g)
a(chn)ڼЧҊ(jin)(bio)
mÃx
ø(bio)x zy(c)L(zhng)450nmL(zhng)630nm
ӱҪ
1. ӱɼҪ(zhn)Ҫܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2Сr(sh)ٌѪƷx3000D(zhun)/x6ϣʹѪ壨{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr(sh)
2. ӱʹѪѪ{ػEDTAȿ(hu)Ӱ푌(sh)(yn)Y(ji)
3. ӱкïBc(hu)Ӱ푌(sh)(yn)ĽY(ji)߶ѪĘӱ](mi)ȫտsѪӱȾĘӱܕ(hu)e(cu)`ĽY(ji)
4. rĘӱ28o(w)l¿ɱһrӱL(zhng)A-20ⷴ(f)ڡ
z(yn)
1. (sh)(yn)(zhn)䣺حh(hun)ȡԇҜƽ30ͬr(sh)sϴҺ120ϡ
2. Ӵy(c)(bio)y(c)(bio)ÿ0.05O(sh)(yng)Ԍ(du)2Ԍ(du)2ף0.05հ(du)1
3. øY(ji)ÿ0.05հ(du)տײֻNz3730
4. ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעM(mn)ÿףo510ȥ׃(ni)ϴҺĸɣ˷(f)5ĸ
2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עM(mn)ÿף_ÿo(w)ˮĸɡ
5. @ɫ ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ15
6. Kֹ(yng) ÿKֹҺ0.05
7. y(c)ø(bio)xx(sh)xβL(zhng)450 nmԿհУ㣩pL(zhng)450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)
(yng)Дֵ
Cutoff ֵӋ(j)㣺 COV = Ԍ(du)ƽODֵ2.1
(bio)ODֵCOV(yng)(bio)ODֵCOV
עԌ(du)ODֵ0.050.05Ӌ(j)㣬0.05(sh)HODֵӋ(j)
z(yn)Y(ji)Ľጡ
1. ԽY(ji)ӱвҸeԭHBeAgӱеҸeԭHBeAgԇеęzy(c)
2. (yng)ԽY(ji)ӱкҸeԭHBeAgخ(yng)
3. (yng)ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)ֻн(jng)^(gu)؏(f)؏(f)(sh)(yn)Ԟ(yng)ԽY(ji)ĘӱJ(rn)ҸeԭHBeAg(yng)ԡ؏(f)Y(ji)(yng)ԵĘӱ(yng)(jng)^(gu)m(dng)?sh)ֶM(jn)д_J(rn)
(yng)ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)(yng)ԽY(ji)܌һ(g)(g)g(sh)(wn)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
z(yn)ľԡ
иԵߌ(sh)(yn)ϵy(tng)ڝڵķخԷ(yng)؏(f)(yng)ԽY(ji)(yng)һ(g)m(dng)?sh)ķM(jn)д_J(rn)
a(chn)Ʒָ(bio)
1 ԇ^Һwo(w)
2 ԅƷʣ15ԅƷzy(c)Y(ji)óF(xin)(yng)
3 (yng)ԅƷʣ10(yng)ԅƷzy(c)Բö1ݡ
4͙z͙zƷy(c)(yng)1IU/mL
5 ԣþԅƷy(c)CV(yng)15n=10
6 (wn)ԇ(yn)ԇM376zY(ji)(yng)_(d)15Ҫ
7 bݘ(bio)ʾb
ע(xing)
1. ƷHw\Hޙzy(c)wѪѪ{zy(c)Y(ji)\ָ(bio)֮һ
2. 28lȡĘӱ(yng)Ҝƽ30M(jn)Йzy(c)
3. 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(hu)(do)e(cu)`Č(sh)(yn)Y(ji)
4. ʹҺֹӘӕr(sh)ÿΑ(yng)ԓQ^ȡӱ
5. õƿμӕr(sh)Ո(qng)JҺwuȥ12κֱٵصμҪ⌢MеҺw|?yn)R߅eøY(ji)
6. ڲ^(gu)Б(yng)Mⷴ(yng)Юa(chn)ݡ
7. sϴҺϵߝ}ܕ(hu)γɽY(ji)δȫܽ⣬(hu)Ӱ푌(sh)(yn)Y(ji)ͬr(sh)ܕ(hu)סԄ(dng)ӘϴC(j)ܵF(xin)Y(ji)Ո(qng)37ܽ⣬Ȼϡጻʹ
8. z؏(f)ʹ
9. ˮԡ偷(yng)r(sh)Ո(qng)(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37档
10. ϴ壺1ϴC(j)ϴr(sh)ϴC(j)ļҺP(gun)ҪȱϴҺ^(gu)ܳM(mn)(yng)УϴΔ(sh)(yng)5(jng)עzҺ^Ƿ
2ֹϴr(sh)Ո(qng)ʹÎмˮİԷԴ^(gu)ø(li)߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zy(c)Y(ji)Ĝ(zhn)_
3ϴr(sh)õˮՈ(qng)(f)ʹ
4ϴC(j)ÿʹǰsˮȥxˮϴɃԷֹ·g
11. κԇ|Ƥw۾ôˮ(du)ԓλM(jn)ДU(ku)ϴKֹҺиg
12. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈ(qng)P(gun)Č(sh)(yn)ҹҎ(gu)(li)(zh)к̎