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Y˷֦Uw(IgG)zyԇ_ϺsʢˎI(y)ɷ޹˾
ԇ
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Y˷֦Uw(IgG)zyԇ

Y˷֦Uw(IgG)zyԇ

aƷQ

ͨQY˷֦UwIgGzyԇУø“(lin)߷


bҎ(gu)

48˷/96 ˷/


aƷڶԙzyѪѪ{нY˷֦Uw(IgG)

aƷzyYHR_\ųΨһELISAzyY˲߿w߀܇^(q)ǽڸȾȾ߀[ԸȾв鯟Ч˵ԷνY˿Y˿w@ڼY˷֦UȾɽM֮gʾ@fѪѪ{нY˿wzybeԷνY˺ͼY˷֦UȾ߾o\rֵ


ƷɻؽM_ĽY˷֦UλںϿԭø“(lin)ӴyѪ{ѪyƷдڽY˿wγɿԭwخYټ뿹IgG ø˿wc䷴ ø@ɫɫ\ДƷнY˿w


ҪMɳɷ֡

1 TB-ԭ                1K 96˷  1K48˷

2 øY                         20mL1ƿ   6mL1ƿ

3 Ԍ                          1mL1ƿ   1mL1ƿ

4 Ԍ                          1mL1ƿ   1mL1ƿ

5 ϴҺǰ120ϡጣ       50mL1ƿ   30mL1ƿ

6 A                           6mL1ƿ        3mL1ƿ

7 B                           6mL2ƿ   3mL1ƿ

8 KֹҺ                           6mL1ƿ   3mL1ƿ

9 ƷϡҺ                     20mL1ƿ   6mL1ƿ

10zƬ                         3              3

ԇMɣ

M2øYNa2HPO4.12H2O 2.9g/LNaCL 8g/LKCL 0.2g/LKH2PO4 0.2g/Lͺø(wn)AES 0.6g/L^øo1.0g/LСţѪ 500mL/LɽѪ 500mL/Lָʾ1 1.3mL/L1g/L-TB-HRPm

M3ԌգNa2HPO4.12H2O 2.9g/LNaCL 8g/LKCL 0.2g/LKH2PO4 0.2g/LҶ 150mL/LСţѪ 250mL/L1g/Lָʾ1 1.25mL/LѪm

M4ԌգNa2HPO4.12H2O 2.9g/LNaCL 8g/LKCL 0.2g/LKH2PO4 0.2g/LҶ150mL/L1g/LѪ250mL/L

M5ϴҺ: NaCL 160g/LNa2HPO4.12H2O 58g/LKCL 4g/LKH2PO4 4g/LTween-20 10mL/L

M6@ɫA8g/L8-u0.004g/L2.4mL/L^0.7g/L

M7@ɫB2.1g/LҶc0.38g/LTMB 0.4g/L

M8KֹҺ110mL/L

M9ƷϡҺTris Base 2.4g/LNaCL 17.8g/Lҵc5g/LţѪ׵2g/Lœ-20 1mL/LͨX-100 1mL/LɽѪ50mL/L0.4g/L

ҪδṩƷrsˮȥxˮһ_΢Һ͝^Ƀˮ37ˮԡ偡ӋrϴCϴƿʎ


lЧڡ

28汣Ч12

aڼЧҊ˺


   mÃx

Ԅø˜yxL450nm


ӱҪ

1ӱɼҪʂҪܙz߽ʳ

2ӱʹѪѪ{ػEDTAȿӰ푌Y

3߶ѪĘӱ]ȫտsѪӱԼ΢ȾĘӱe`ĽYɌ¼

4MܲrѪzyӱҜ²Ҫ^8Сr

58СrəzyՈӱ28ر棻48СrəzyҪӱM\ݔՈӱ-20͜ض

6ӱڽ֮zyǰ횳ֻ򣻘ӱⷴ̓tɘӱIgG׾ۺϣɌ¼ҲɌԙz½


z򞷽

1ÿԇOԌոɿքeԌѪ100LOһ׿հ׌ӘƷϡҺ100L׼100LƷϡҺټӴyѪ10L3730ȥ׃ȘƷ۸ɡ

2ϴҺעMÿף300LϴҺ/ףo5犺۸ɣ؏5

3ÿ׼øY100Lհ׌տ׳⣩3720犣ͬϷϴ5

4ÿ׼@ɫAҺBҺ50Lppʎ37ܹo10

5ÿ׼KֹҺ50LKֹԿհ{ø˃xxȡOD450


zYĽጡ

1yø˃xβL450nm pL450nm/630nmyODֵÆβLyrOհ׌տ

ԌÿODֵ0.80.00< ԌODֵ0.12

2RֵCutoffӋ㣺ԌƽODֵ0.12

עԌODֵ0.060.06Ӌ0.06HֵӋ㡣

(3) Y:ƷODֵRֵCutoffY˿wƷODֵRֵCutoffY˿w


z򞷽ľԡ

ƷHڙzyѪѪ{нY˿wwzyY˲o\


aƷָˡ

1 Y˷֦UwI(y)ƷzY

 

ԅƷ

ԅƷ

`ȅƷ

TBƷ

90%

100%

ϸ

2R400˱zyY˷֦UwzyY

νY˲˘˱122+104/252100%=89.68%

YĤײ˘˱7+15/27100%=81.48%

Y˲˘˱2+13/21100%=71.4%

жȲ˘˱122+104+7+15+2+13/(252+27+21)100%=87.67%

خԣ100-7/100100%=93%

ӱʣ263+93/400100%=89.00%

3zyԇЌLP(ji)tǯנٮԼβȾߘӱMМyԇ^cP淴ԡY163Ʒмʞ8%cīI4.6-11.3%һfԇcһĽ淴ҲcԇڽY˲߶Эh(hun)w[ԸȾPĿǰ]аl(f)؄eǷβȾ

4Ҫɔ_Ѫ֬Ⱥ2.1mmoL/Lđ̴đ̴Ⱥ8.5mmoL/LѪtףѪtםȲ210g/LƷĸɔ_ԇYNɔ_Ӱ푱ƷęzyY


ע헡

1sϴҺʹǰsˮϡ20ʹ

2̖ͬԇɻʹ

3횇ƜĜضȺ͕rg

4Yжԃxyܑ{^