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͸ײIgMwzyԇУø“(lin)߷_ϺsʢˎI(y)ɷ޹˾
ԇ
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͸ײIgMwzyԇУø“(lin)߷

͸ײIgMwzyԇУø“(lin)߷

a(chn)ƷQ

ͨQ͸ײIgMwzyԇУø“(lin)߷


bҎ(gu)

48˷/96˷/С


A(y);

mڶԜyѪѪ{м͸ײIgMw-HAVIgM

ڼ͸׵o\


ԇϵÿIgM()΢װ͸(HAV)ԭøӛĿ͸ײ(-HAV)ԇƳ(yng)ò@ԭzyѪӱеļ׸IgMw-HAVIgMڼ͸׵o\


ҪMɳɷ֡

ԇнM

48˷/

96˷/

1.A(y)(yng)

48/K1K

96/K1K

2.øY(ji)

3mL1ƿ

6mL1ƿ

3.Ԍ

0.5mL1ƿ

0.5mL1ƿ

4.Ԍ

0.5mL1ƿ

0.5mL1ƿ

5.sϴҺ

30mL1ƿ

30mL1ƿ

6.@ɫA

3mL1ƿ

6mL1ƿ

7.@ɫB  

3mL1ƿ

6mL1ƿ

8.KֹҺ2M H2SO4

3mL1ƿ

6mL1ƿ

9. ׸οԭ

3mL1ƿ

6mL1ƿ

10.z

11.Է

ע⣺̖ͬԇԇнM12 ɻá

MݽMɣ

øY(ji)Na2HPO412H2O 2.9g/LKH2PO4 0.2g/LKCl 0.2g/LNaCl 8g/LҶ 150mL/LСţѪ 500mL/Lָʾ1 1.25mL/L 1g/L-HAV-HRP m

ԌգNa2HPO412H2O  2.9g/LNaCl  8g/LKCl  0.2g/LKH2PO4  0.2g/LҶ150mL/LСţѪ250mL/L1g/LѪ m

ԌգNa2HPO4•12H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/L 2g/LҶ150mL/L 1g/LѪ 250mL/L

A 8g/LԇA 0.004g/LԇB 2.4mL/L^ 0.7g/L

B 2.1g/LҶc 0.38g/LTMB 0.4 g/L

KֹҺ2M H2SO4

׸οԭ׸οԭ1000mL/LţѪ׵ 1g/Lָʾ2 1mL/L

ҪδṩƷ

1. rsˮȥxˮ

2. һ

3. _΢Һ͝^

4. Ƀˮ

5. 37ˮԡ

6. Ӌr

7. ϴCϴƿC

8. ʎ


lЧڡ

  ԇБ(yng)28бܹⱣЧ12

a(chn)ڼЧҊ˺

mÃx

ø˃x zyL450nmL630nm


ӱҪ

1. ӱɼҪʂҪܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2СrٌѪƷxģ3000D(zhun)/x6ʹѪ壨{OٺѪ鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr

2. ӱʹѪѪ{ػEDTAȿӰ푌Y(ji)

3. ӱкïBcӰ푌ĽY(ji)߶ѪĘӱ]ȫտsѪӱ΢ȾĘӱܕe`ĽY(ji)

4. rĘӱ28ol¿ɱһrӱLA-20£ⷴ(f)

z򞷽

1. ʂ䣺حh(hun)ȡԇҜƽ30犣ͬrsϴҺ1:20ϡ

2Әӣ˱}ˮ11000ϡጺҺȡ100΢뷴(yng)׃(ni)O(sh)Ԍ2ÿ100΢Ԍ2ףÿ100΢հ׌1÷Ƭw(yng)3730犡

3ϴ죺ȥ׃(ni)ҺwϴҺעMÿo30˦(f)5۸

4ͬrHAVAgøY(ji)50΢հ׌տײֻ÷Ƭw(yng)3720

5؏(f)3ϴ^

6@ɫÿ׵μ@ɫAB50΢3710

7ӽKֹҺÿ׵μӽKֹҺ50΢

8. yø˃xx(sh)xβL450 nmԿհ׿У㣩pL450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)


Дֵ

ø˃xx(sh)L450nmÿհ׿УcȻxȡODֵ

עԌODֵ0.050.05Ӌ0.05HODֵӋ

zY(ji)Ľጡ

1. ԽY(ji)ӱв׸IgMw-HAVIgMӱеļ׸IgMw-HAVIgMԇеęzy

2. ԽY(ji)ӱкм׸IgMw-HAVIgMخ(yng)

3. ԽY(ji)ĘӱҪM؏(f)򞣬ֻн(jng)^؏(f)؏(f)ԞԽY(ji)ĘӱJǼ׸IgMw-HAVIgM؏(f)Y(ji)ԵĘӱ(yng)(jng)^m?sh)ֶMд_J

ԽY(ji)Ęӱ(f)zԽY(ji)(yng)JԘӱ؏(f)ԽY(ji)܌һg(sh)}£

1 ڃxӘɵĽȾ

2 @ɫxȾ

3 ԇ©ɵĽȾ

4 ֵϴȥص


z򞷽ľԡ

   иԵߌϵy(tng)ڝڵķخԷ(yng)؏(f)ԽY(ji)(yng)һm?sh)ķMд_J


a(chn)Ʒָˡ

1.ԇ^͸Һwo

2.ԅƷʣ10ԅƷzyY(ji)óF(xin)

3.͙zޣ͙zޅƷzy(yng)1:16

4.ԣþԅƷyCV(yng)15n=10

5.(wn)ԇ򞣺ԇM376zY(ji)(yng)_2.12.5Ҫ

6.bݘʾb


ע헡

1. ƷHw\HޙzywѪ塢Ѫ{zyY(ji)\ָ֮һ

2. 28lȡĘӱ(yng)Ҝƽ30MЙzy

3. 횴_ƷӘĜʴ_ӘӲʴ_ܕ(do)e`ČY(ji)

4. ʹ΢ҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ

5. õƿμӕrՈJҺwuȥ12κֱٵصμҪ⌢MеҺw|R΢߅؄eøY(ji)

6. ڲ^Б(yng)Mⷴ(yng)΢Юa(chn)ݡ

7. sϴҺϵߝ}ܕγɽY(ji)δȫܽӰ푌Y(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽ⣬Ȼϡጻʹ

8. z؏(f)ʹ

9. ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37档

10. ϴ壺1ϴCϴrϴCļҺP(gun)ҪȱϴҺ^ܳM(yng)΢ϴΔ(sh)(yng)5(jng)עzҺ^Ƿ

2ֹϴrՈʹÎмˮİ壬ԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝʴ_


3ϴrõˮՈ𷴏(f)ʹá

4ϴCÿʹǰsˮȥxˮϴɃԷֹ·g

11. κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиgԡ

12. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎