a(chn)ƷQ
ͨQײIgMwzyԇУø(lin)߷
bҎ(gu)
48˷/96˷/С
A(y);
mڶԜyѪѪ{мײIgMw-HAVIgM
ڼo\
zԭ
ԇϵÿIgM()װ(HAV)ԭøӛĿײ(-HAV)ԇƳ(yng)ò@ԭzyѪӱеļIgMw-HAVIgMڼo\
ҪMɳɷ֡
ԇнM |
48˷/ |
96˷/ |
1.A(y)(yng) |
48/K1K |
96/K1K |
2.øY(ji) |
3mL1ƿ |
6mL1ƿ |
3.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
4.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
5.sϴҺ |
30mL1ƿ |
30mL1ƿ |
6.@ɫA |
3mL1ƿ |
6mL1ƿ |
7.@ɫB |
3mL1ƿ |
6mL1ƿ |
8.KֹҺ2M H2SO4 |
3mL1ƿ |
6mL1ƿ |
9. οԭ |
3mL1ƿ |
6mL1ƿ |
10.z |
2Ƭ |
2Ƭ |
11.Է |
1ֻ |
1ֻ |
ע⣺̖ͬԇԇнM12 ɻá
MݽMɣ
øY(ji)Na2HPO412H2O 2.9g/LKH2PO4 0.2g/LKCl 0.2g/LNaCl 8g/LҶ 150mL/LСţѪ 500mL/Lָʾ1 1.25mL/L 1g/L-HAV-HRP m
ԌգNa2HPO412H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/LҶ150mL/LСţѪ250mL/L1g/LѪ m
ԌգNa2HPO4•12H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/L 2g/LҶ150mL/L 1g/LѪ 250mL/L
@ɫA 8g/LԇA 0.004g/LԇB 2.4mL/L^ 0.7g/L
@ɫB 2.1g/LҶc 0.38g/LTMB 0.4 g/L
KֹҺ2M H2SO4
οԭοԭ1000mL/LţѪ 1g/Lָʾ2 1mL/L
ҪδṩƷ
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌr
7. ϴCϴƿC
8. ʎ
lЧڡ
ԇБ(yng)28бܹⱣЧ12
a(chn)ڼЧҊ˺
mÃx
ø˃x zyL450nmL630nm
ӱҪ
1. ӱɼҪʂҪܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2СrٌѪƷxģ3000D(zhun)/x6ʹѪ壨{OٺѪ鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr
2. ӱʹѪѪ{ػEDTAȿӰ푌Y(ji)
3. ӱкïBcӰ푌ĽY(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY(ji)
4. rĘӱ28ol¿ɱһrӱLA-20£ⷴ(f)
z
1. ʂ䣺حh(hun)ȡԇҜƽ30犣ͬrsϴҺ1:20ϡ
2Әӣ˱}ˮ11000ϡጺҺȡ100뷴(yng)׃(ni)O(sh)Ԍ2ÿ100Ԍ2ףÿ100հ1÷Ƭw(yng)3730犡
3ϴ죺ȥ׃(ni)ҺwϴҺעMÿo30˦(f)5۸
4ͬrHAVAgøY(ji)50հտײֻ÷Ƭw(yng)3720
5؏(f)3ϴ^
6@ɫÿμ@ɫAB503710
7ӽKֹҺÿμӽKֹҺ50
8. yø˃xx(sh)xβL450 nmԿհУ㣩pL450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)
Дֵ
ø˃xx(sh)L450nmÿհУcȻxȡODֵ
עԌODֵ0.050.05Ӌ0.05HODֵӋ
zY(ji)Ľጡ
1. ԽY(ji)ӱвIgMw-HAVIgMӱеļIgMw-HAVIgMԇеęzy
2. ԽY(ji)ӱкмIgMw-HAVIgMخ(yng)
3. ԽY(ji)ĘӱҪM؏(f)ֻн(jng)^؏(f)؏(f)ԞԽY(ji)ĘӱJǼIgMw-HAVIgM؏(f)Y(ji)ԵĘӱ(yng)(jng)^m?sh)ֶMд_J
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)JԘӱ؏(f)ԽY(ji)܌һg(sh)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
zľԡ
иԵߌϵy(tng)ڝڵķخԷ(yng)؏(f)ԽY(ji)(yng)һm?sh)ķMд_J
a(chn)Ʒָˡ
1.ԇ^Һwo
2.ԅƷʣ10ԅƷzyY(ji)óF(xin)
3.͙zޣ͙zޅƷzy(yng)1:16
4.ԣþԅƷyCV(yng)15n=10
5.(wn)ԇԇM376zY(ji)(yng)_2.12.5Ҫ
6.bݘʾb
ע헡
1. ƷHw\HޙzywѪ塢Ѫ{zyY(ji)\ָ֮һ
2. 28lȡĘӱ(yng)Ҝƽ30MЙzy
3. 횴_ƷӘĜʴ_ӘӲʴ_ܕ(do)e`ČY(ji)
4. ʹҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ
5. õƿμӕrՈJҺwuȥ12κֱٵصμҪ⌢MеҺw|R߅eøY(ji)
6. ڲ^Б(yng)Mⷴ(yng)Юa(chn)ݡ
7. sϴҺϵߝ}ܕγɽY(ji)δȫܽӰ푌Y(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽ⣬Ȼϡጻʹ
8. z؏(f)ʹ
9. ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37档
10. ϴ壺1ϴCϴrϴCļҺP(gun)ҪȱϴҺ^ܳM(yng)ϴΔ(sh)(yng)5(jng)עzҺ^Ƿ
2ֹϴrՈʹÎмˮİ壬ԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝʴ_
3ϴrõˮՈ(f)ʹá
4ϴCÿʹǰsˮȥxˮϴɃԷֹ·g
11. κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиgԡ
12. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎