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͸ײĿԭzyԇУø“(lin)߷_ϺsʢˎI(y)ɷ޹˾
ԇ
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͸ײĿԭzyԇУø“(lin)߷

͸ײĿԭzyԇУø“(lin)߷

a(chn)ƷQ

ͨQ:͸ײĿԭzyԇУø“(lin)߷


bҎ(gu)

96˷/


A(y);

a(chn)ƷwⶨԙzyѪѪ{еı͸ײxĿԭ

pwAø“(lin)߷ԭELISAԙzyѪѪ{Ʒеı͸ײxĿԭozy

͸ײHepatitis C VirusHCVݔѪ󲡶ԷǼ׷͸׵ҪԭҪͨ^ݔѪo}Ҳͨ^н|ĸĿǰȫs1.31.7|˞ԱβȾÿ35fc͸P(gun)ĸK(j)Ӌÿİf˸Ⱦβ

βȾЕr(do)¼԰Yćس̶ȿȾβпֻF(xin)p΢YҲܽKЇԸβ܌(do)¸Ӳ͸ΰȾs80%˲F(xin)κΰYܳF(xin)ļ԰Yl(f)ᡢȫʳ½Iʹɫɫ׃\P(gun)(ji)ʹS㣨Ƥw۰װl(f)SԸȾ߳F(xin)Yܱ仼ڸβһ15~20%ҵHCVȾߞԸȾ^80~85%ĸȾ߰l(f)չԱ͸20%Ŀɰl(f)չwSK4~5%ĸwS߰l(f)μԸΰΣʮևء͸ɱβĸβ

͸׺Ŀԭzy½l(f)չһNHCVȾęzyڸȾHCVHCVĿԭF(xin)^磬cѪHCV RNAdP(gun)HCVȾ򿵏(f)ʧHCVȾ\ࡢIѪTYHCVȾ\ΣȺO(jin)y߹ϵKߵHCVȾ\beȾֻF(xin)YȾڿЧA(y)yͱO(jin)y


ƷɻؽM_ı͸ײą^(q)ԭHCV-cAgСƂĆο¡w΢װøӛHCV-cAgο¡wԡԌѪȽM(yng)pwAø“(lin)߷ԭELISAzyѪѪ{Ʒб͸ײxĿԭ


ҪMɳ

1

w

96˷1K

2

øY(ji)

20mL1ƿ

3

ԌѪ

0.5 mL1ƿ

4

ԌѪ

0.5 mL1ƿ

5

ϴҺʹÕr20ϡጣ

50mL1ƿ

6

 A

6mL2ƿ

7

 B

6mL2ƿ

8

KֹҺ

6mL1ƿ

9

ƷϡҺ

10mL1ƿ

10

3

ע⣺̖ͬĽMֲû


MҪMɣ1w壺ᘌĿԭĆο¡w2øY(ji)u׻龏_ҺɽѪţѪ׵3ԌѪ͸ײĿԭzyѪ壻4ԌѪ͸ײĿԭzyԵѪ5 ϴҺNaClͨ X-1006Aˮc^أ7BҶļ׻“(lin)8KֹҺ9ƷϡҺ Tris _ҺNaClҵcţѪ׵ףœ-20ͨX-100ɽѪ  


Ʒ̓x

1rƂsˮȥxˮ

2һף

3_΢Һ͝^

4Ƀˮ

537ˮԡ䣻

6Ӌr

7ϴCϴƿ

8ʎ

9ø˃x


28ܹⱣ棬Ч12

a(chn)ڼЧҊ˺


mÃx

Ԅø˜yxpLrO(sh)L450nmL630nmβLrO(sh)ÙzyL450nmO(sh)ÿհ׌{(dio)


ӱҪ

    1ӱʹѪѪ{cػEDTAȿӰ푌Y(ji)

2ӱoƂ̎_t(y)üg(sh)ɼȫѪӱoðСrϺ3000D(zhun)/x10ַxѪʹѪ岻Oٺt׼tܕ(do)¼ԽY(ji)

3ʹüӟ̎Ęӱ֬Ѫӱ(yng)ڌǰóxķӱ

4SȾĘӱ(do)e`ĽY(ji)

5ӱкЯBcӰ푌Y(ji)ïBcӱ

6ѪӱδǰxČ(do)wS׵ĴڣwS׌Y(ji)Ӱ횴_x̎ǰӱѽ(jng)ڽܿίIJ˘ӱҪLrg


z򞷽 

1ʂ

1حh(hun)ȡԇУҜƽ30

2ϴҺƣԇеϴҺsϴҺںmĝ(ni)rƂsˮȥxˮsϴҺϡ20

sϴҺߝ}_Һڜضȵ͕rܕнY(ji)Ոʹǰ_sϴҺoY(ji)l(f)F(xin)нY(ji)ՈˮԡмӟܽtӰϴЧ

2

1ÿΌڰO(sh)һհ׿O(sh)ꎡԌոp

2ԌѪO(sh)ɿמԌÿ׼ԌѪ200L

3ԌѪO(sh)ɿמԌգÿ׼ԌѪ200L

4ӘƷϡҺÿףԌտףм100LƷϡҺհ׿׼200LƷϡҺ

5ӘӣƷÿ׼ƷѪѪ{100L

6òzƬ371ԡʎ60

7ϴ죺СĽҵĤȥ(yng)l׃(ni)ҺwϴҺҺעMÿףÿב(yng)벻400LϴҺҺo510ȥ׃(ni)ϴҺ؏(f)5ڸɃˮĸ

8øY(ji)ÿ׼200LøY(ji)

9òzƬ371ԡ30

10ϴ죺ͬ7

11@ɫÿȼ100LAټ100LBֻ371ԡܹ@ɫ15

12Kֹ(yng)ÿ׼50LKֹҺֻ

13 Y(ji)yY(ji)ڷ(yng)Kֹ10犃(ni)MМyʹø˃xyֵxβL450nmO(sh)հ׿{(dio)㣩xpLL450nmL630nmODֵ


Дֵ

CUT OFF_Ԍƽֵ+0.06ԌODֵ0.06r0.06Ӌ㣩

ԌÿODֵ>0.8ԌODֵÿף0.1ryY(ji)Ч

Y(ji)ж

1ODֵCUT OFFжHCVĿԭ

2ODֵRֵCUT OFFжHCVĿԭ

zԵĘƷ(yng)Mp׏(f)zԴ_J挍Y(ji)


zY(ji)Ľ

1ѪӱδȫѪ{ӱwS׻ӱȾY(ji)Ӱ푡

2ʹLrgA򷴏(f)ڵĘӱԇęzy`Ӱ푣hʹrӱMЌ


z򞷽ľ

1ԇЃHڶԙzyѪѪ{ƷеHCVxĿԭ

2ELISA(yng)ԭԇzyY(ji)ԲȫųHCVȾĿܣzyY(ji)Ա횽Y(ji)ϴ_Cԇָ˺RϢMз


a(chn)Ʒָˡ

15I(y)HCVĿԭԅѪzyY(ji)óF(xin)ԣ4݇HCVĿԭԅѪzyԅƷʣ+/+(yng)3/4

10I(y)HCVĿԭԅѪzyY(ji)óF(xin)4I(y)͙zޅƷzyS1S2 S3(yng)S45͙݇zޅƷzyL1L2 L3(yng)L4pL5Ի   

I(y)ҾԅƷ؏(f)10Ιzyп׌Y(ji)CV%15%n=10

(wn)ԇM376ָ˾(yng)Ҏ(gu)

2ԇzy͸ײĿԭ`Ȟ1pg/mL

3淴(yng)HBVHIVHAVԘӱӰ푱ԇЌHCVĿԭęzy25RFߝԘӱ23ANAߝԘӱքeñԇMHCVĿԭzyӱԔ(sh)1خԷքe96.0%95.7%

4Rо60ͬԴѪͬһߵѪѪ{zyY(ji)ȫһ


ע헡

128lȡĘӱ(yng)Ҝƽ30MЙzy

2횴_ӘĜʴ_ӘӲʴ_ܕ(do)e`ČY(ji)

3ʹ΢ҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ

4ڲ^Б(yng)M΢Юa(chn)ݡ

5sϴҺϵߝ}ܕγɽY(ji)δȫܽӰ푌Y(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽȻϡጾʹ

6z؏(f)ʹ

7ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧Σˮؿ37

8ϴ壺1ϴCϴrϴCļҺP(gun)ҪϴҺ^ܳM(yng)΢ϴΔ(sh)(yng)5Σ(jng)עzҺ^Ƿ

2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝʴ_

3ϴrõˮՈ𷴏(f)ʹá

4ϴCÿʹǰsˮȥxˮ_ϴɃԷֹ·g

9κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиgԡ

10ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎