a(chn)ƷQ
ͨQ:ײĿԭzyԇУø(lin)߷
bҎ(gu)
96˷/
A(y);
a(chn)ƷwⶨԙzyѪѪ{еıײxĿԭ
pwAø(lin)߷ԭELISAԙzyѪѪ{ƷеıײxĿԭozy
ײHepatitis C VirusHCVݔѪԷǼҪԭҪͨ^ݔѪo}Ҳͨ^н|ĸĿǰȫs1.31.7|˞ԱβȾÿ35fcP(gun)ĸK(j)Ӌÿİf˸Ⱦβ
βȾЕr(do)¼Yćس̶ȿȾβпֻF(xin)pYҲܽKЇԸβ܌(do)¸ӲΰȾs80%˲F(xin)κΰYܳF(xin)ļYl(f)ᡢȫʳ½Iʹɫɫ׃\P(gun)(ji)ʹS㣨Ƥw۰װl(f)SԸȾ߳F(xin)Yܱ仼ڸβһ15~20%ҵHCVȾߞԸȾ^80~85%ĸȾ߰l(f)չԱ20%Ŀɰl(f)չwSK4~5%ĸwS߰l(f)μԸΰΣʮևءɱβĸβ
Ŀԭzy½l(f)չһNHCVȾęzyڸȾHCVHCVĿԭF(xin)^磬cѪHCV RNAdP(gun)HCVȾ(f)ʧHCVȾ\ࡢIѪTYHCVȾ\ΣȺO(jin)y߹ϵKߵHCVȾ\beȾֻF(xin)YȾڿЧA(y)yͱO(jin)y
zԭ
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ҪMɳ
1 |
w |
96˷1K |
2 |
øY(ji) |
20mL1ƿ |
3 |
ԌѪ |
0.5 mL1ƿ |
4 |
ԌѪ |
0.5 mL1ƿ |
5 |
ϴҺʹÕr20ϡጣ |
50mL1ƿ |
6 |
@ɫ A |
6mL2ƿ |
7 |
@ɫ B |
6mL2ƿ |
8 |
KֹҺ |
6mL1ƿ |
9 |
ƷϡҺ |
10mL1ƿ |
10 |
zƬ |
3 |
ע⣺̖ͬĽMֲû
MҪMɣ1w壺ᘌĿԭĆο¡w2øY(ji)u龏_ҺɽѪţѪ3ԌѪײĿԭzyѪ壻4ԌѪײĿԭzyԵѪ5 ϴҺNaClͨ X-1006@ɫAˮc^أ7@ɫBҶļ(lin)8KֹҺ9ƷϡҺ Tris _ҺNaClҵcţѪף-20ͨX-100ɽѪ
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4SȾĘӱ(do)e`ĽY(ji)
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6ѪӱδǰxČ(do)wSĴڣwSY(ji)Ӱ횴_x̎ǰӱѽ(jng)ڽܿίIJ˘ӱҪLrg
z
1ʂ
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2ϴҺƣԇеϴҺsϴҺںmĝ(ni)rƂsˮȥxˮsϴҺϡ20á
sϴҺߝ}_Һڜضȵ͕rܕнY(ji)Ոʹǰ_sϴҺoY(ji)l(f)F(xin)нY(ji)ՈˮԡмӟܽtӰϴЧ
2
1ÿΌڰO(sh)һհO(sh)ꎡԌոp
2ԌѪO(sh)ɿמԌÿԌѪ200L
3ԌѪO(sh)ɿמԌգÿԌѪ200L
4ӘƷϡҺÿףԌտףм100LƷϡҺհ200LƷϡҺ
5ӘӣƷÿƷѪѪ{100L
6òzƬ371ԡʎ60犡
7ϴ죺СĽҵĤȥ(yng)l׃(ni)ҺwϴҺҺעMÿףÿב(yng)벻400LϴҺҺo510ȥ׃(ni)ϴҺ؏(f)5ڸɃˮĸ
8øY(ji)ÿ200LøY(ji)
9òzƬ371ԡ30
10ϴ죺ͬ7
11@ɫÿȼ100L@ɫAټ100L@ɫBֻ371ԡܹ@ɫ15
12Kֹ(yng)ÿ50LKֹҺֻ
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Дֵ
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ԌÿODֵ>0.8ԌODֵÿף0.1ryY(ji)Ч
Y(ji)ж
1yƷODֵRֵCUT OFFжHCVĿԭ
2yƷODֵRֵCUT OFFжHCVĿԭ
zԵĘƷ(yng)Mp(f)zԴ_J挍Y(ji)
zY(ji)Ľ
1ѪӱδȫѪ{ӱwSӱȾY(ji)Ӱ푡
2ʹLrgA(f)ڵĘӱԇęzy`Ӱ푣hʹrӱMЌ
zľ
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a(chn)Ʒָˡ
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I(y)ҾԅƷ؏(f)10ΙzyпY(ji)CV%15%n=10
(wn)ԇM376ָ˾(yng)Ҏ(gu)
2ԇzyײĿԭ`Ȟ1pg/mL
3淴(yng)HBVHIVHAVԘӱӰ푱ԇЌHCVĿԭęzy25RFߝԘӱ23ANAߝԘӱքeñԇMHCVĿԭzyӱԔ(sh)1خԷքe96.0%95.7%
4Rо60ͬԴѪͬһߵѪѪ{zyY(ji)ȫһ
ע헡
128lȡĘӱ(yng)Ҝƽ30MЙzy
2횴_ӘĜʴ_ӘӲʴ_ܕ(do)e`ČY(ji)
3ʹҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ
4ڲ^Б(yng)MЮa(chn)ݡ
5sϴҺϵߝ}ܕγɽY(ji)δȫܽӰ푌Y(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽȻϡጾʹ
6z؏(f)ʹ
7ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧Σˮؿ37
8ϴ壺1ϴCϴrϴCļҺP(gun)ҪϴҺ^ܳM(yng)ϴΔ(sh)(yng)5Σ(jng)עzҺ^Ƿ
2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝʴ_
3ϴrõˮՈ(f)ʹá
4ϴCÿʹǰsˮȥxˮ_ϴɃԷֹ·g
9κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиgԡ
10ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎