a(chn)ƷQ
ͨQh(hun)ϰĿw(CCP) zyԇ ø(lin)߷
bҎ(gu)
48 ˷/96 ˷/
A(y);
ԓa(chn)Ʒt(y)C(j)(gu)ڶԜyѪҺпh(hun)ϰĿw(CCP)yѪҺпh(hun)ϰĿw(CCP) o\
z(yn)ԭ
ƷgELISAzyCCPwԭǣĿԭh(hun)ϰģCCPø(bio)棬ϡጺĴzѪ/Ѫ{͌뷴(yng)zѪ/Ѫ{дڿh(hun)ϰĿw(jng)tѪ/Ѫ{пh(hun)ϰĿwc(yng)е CCPԭĽY(ji)ϣγɹԭ-w(f)ϴȥδY(ji)ϵѪ/Ѫ{ɷø(bio)ӛĿIgGwCCPԭY(ji)ϿCCPwcø(bio)ӛĿIgGwY(ji)ϴȥδY(ji)ϵø(bio)ӛwɷ֣øɞɫa(chn)(j)ɫ\Ɍh(hun)ϰĿwM(jn)жԜy
ҪMɳɷ֡
øY(ji)Na2HPO4.12H2ONaClKClKH2PO4СţѪɽѪ壻ָʾ1ø(bio)IgG
ƷϡҺTris BaseNaClҵcţѪף-20ͨX-100ɽѪ
CCPԌռУƷϡҺNa2HPO4.12H2ONaClKClKH2PO4ҶСţѪָʾ1
CCPԌգ Na2HPO4.12H2ONaClKClKH2PO4ҶѪָʾ2
ԌգCCPԌռУƷϡҺѪ
ԌգCCPԌռУƷϡҺѪ
@ɫA^
@ɫBҶcTMB
KֹҺ
ϴҺ Na2HPO4.12H2ONaClKClKH2PO4-20
ҪMɣ
1 |
CCPԭ |
1K 96˷ |
|
1K48˷ |
2 |
øY(ji) |
1ƿ10ml |
|
1ƿ5ml |
3 |
Ԍ |
1ƿ1ml |
|
1ƿ1ml |
4 |
Ԍ |
1ƿ1ml |
|
1ƿ1ml |
5 |
ϴҺǰ120ϡጣ |
1ƿ50ml |
|
1ƿ50ml |
6 |
@ɫ A |
1ƿ6ml |
|
1ƿ3ml |
7 |
@ɫB |
1ƿ6ml |
|
1ƿ3ml |
8 |
KֹҺ |
1ƿ6ml |
|
1ƿ3ml |
9 |
ƷϡҺ |
2ƿ50ml |
|
1ƿ50ml |
10 |
|
1ƿ1ml |
|
1ƿ1ml |
11-15 |
УƷϵ |
5ƿ*1ml |
|
5ƿ*1ml |
16 |
f |
1 |
|
1 |
17 |
zƬ |
3 |
|
3
|
lЧڡ
l2-8汣Чڃ(ni)ʹ
Чڣ12 (g)
a(chn)ڼʧЧҊ(bio)
mÃx
Ԅ(dng)ø(bio)yxL450nmL630nm
ӱҪ
ѪѪ{o}Ѫxī@ܷ(f),(bio)2-8ɱһ
z(yn)
1ÿԇ(yn)O(sh)հһףӘƷø(bio)ԇͬԌԌԌոɿzyr(sh)߀O(sh)УƷ5(g)ȸһyӱØƷϡҺ1100ϡጺ100lװ3760犡ȥ׃(ni)Ʒ۸ɡ
2ϴҺעMÿף400lϴҺ/ףo5犺۸؏(f)5
3ÿքe100løY(ji)Ҝط30ͬϷϴ(yng)5Ρ
4ÿ@ɫAҺBҺ50lppʎ37ܹo15
5ÿKֹҺ50lKֹ(yng)Կհ{(dio)ø(bio)xxȡOD450
z(yn)Y(ji)Ľጡ
1 ڶԜy
(1) Y(ji)Ӌ(j)㣺yֵպ͘ƷpȥհֵȻӋ(j)㌦պ͘ƷĽ^ȱֵ
ջƷֵ(OD450)
ȱֵ=-----------------------------
Ԍֵ(OD450)
2Y(ji)Дࣺ |
ȱֵ |
Y(ji) |
|
< 0.95 |
|
|
0.95,1.0 |
߅ֵhy |
|
>1.0 |
|
2ڶ\ࣺ
1Y(ji)Ӌ(j)㣺5(g)УƷУƷA 100 RU/mlУƷB 50 RU/mlУƷC 25 RU/mlУƷD 12 RU/mlУƷE 6 RU/mlȣLog(sh)X-SȣY-S(bio)(zhn)(j)Ѫп-ccpwĝӋ(j)C(j)MϷʽLƘ(bio)(zhn)ą(sh)(sh)兢(sh)(sh)splineM(sh)/(sh)lim/limitѪӱȳУƷA100RU/ml,\Y(ji)Ɉ(bo)飾100RU/mlͬr(sh)hxһ(g)m(dng)?sh)ϡጱ?sh)Ѫϡጺٜy1400Ę(bio)(zhn)Ӌ(j)Ĕ(sh)(j)4ӱccpw
2Y(ji)Дࣺ12RU/ml
12RU/ml
z(yn)ľԡ
ƷHwzyѪѪ{пh(hun)ϰĿw(CCP)L(fng)P(gun)(ji)ײ˵o\
a(chn)Ʒָ(bio)
1ﰲȫԣƂԌƷԌƷԌƷѪ轛(jng)ﰲȫԙzyײ濹ԭzyײwzyȱݲHIV-1/2wzy
2ԇ^ҺwM֑(yng)Һw
3bԇbݘ(bio)ʾb
4ԇհȣ0.05
5ԇ`ȣyԇЃ(ni)У(zhn)ҺEஔ(dng)6RU/mlȷ0.06-0.16
6(zhn)_ȣyԇЃ(ni)У(zhn)ҺBஔ(dng)50RU/mlȷ1.0-2.0
7Է6100RU/ml(ni)Ӌ(j)-(yng)P(gun)ϵ(sh)r0.95
8ԅƷʣ10ԅƷy(yng)ȫz
9ԅƷʣ10ԅƷy(yng)ȫz
10ԣþԅƷy,CV15n=10
11(wn)ԇ(yn)3762-812(g)zY(ji)(yng)_(d)2410(bio)(zhn)
ע(xing)
1sϴҺʹǰsˮϡ20ʹ
2̖ͬԇɻʹ
3횇(yn)Ɯ(yng)ĜضȺ͕r(sh)g
4Y(ji)жԃxy(zhn)ܑ{^
5(dng)ԌODֵ1.80ԌOD0.12r(sh)(sh)(yn)Y(ji)Чġ
t(y)еa(chn)I(y)SC̖ʳˎO(jin)еa(chn)S20030955̖
t(y)еעC̖/a(chn)Ʒg(sh)Ҫ̖
f˜(zhn)ڼڡ