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h(hun)ϰĿw(CCP)zyԇУø“(lin)߷_ϺsʢˎI(y)ɷ޹˾
ԇ
\ \

h(hun)ϰĿw(CCP)zyԇУø“(lin)߷

h(hun)ϰĿw(CCP)zyԇУø“(lin)߷

a(chn)ƷQ

ͨQh(hun)ϰĿw(CCP) zyԇ ø“(lin)߷


bҎ(gu)

48 ˷/96 ˷/

A(y);

ԓa(chn)Ʒt(y)C(j)(gu)ڶԜyѪҺпh(hun)ϰĿw(CCP)yѪҺпh(hun)ϰĿw(CCP) o\


z(yn)ԭ

ƷgELISAzyCCPwԭǣĿԭh(hun)ϰģCCPø(bio)棬ϡጺĴzѪ/Ѫ{͌뷴(yng)zѪ/Ѫ{дڿh(hun)ϰĿw(jng)tѪ/Ѫ{пh(hun)ϰĿwc(yng)е CCPԭĽY(ji)ϣγɹ࿹ԭ-w(f)ϴȥδY(ji)ϵѪ/Ѫ{ɷø(bio)ӛĿIgGwCCPԭY(ji)ϿCCPwcø(bio)ӛĿIgGwY(ji)ϴȥδY(ji)ϵø(bio)ӛwɷ֣ø߻ɞɫa(chn)(j)ɫ\Ɍh(hun)ϰĿwM(jn)жԜy

ҪMɳɷ֡

øY(ji)Na2HPO4.12H2ONaClKClKH2PO4СţѪɽѪ壻ָʾ1ø(bio)IgG

ƷϡҺTris BaseNaClҵcţѪ׵ףœ-20ͨX-100ɽѪ

CCPԌռУƷϡҺNa2HPO4.12H2ONaClKClKH2PO4ҶСţѪָʾ1

CCPԌգ Na2HPO4.12H2ONaClKClKH2PO4ҶѪָʾ2

ԌգCCPԌռУƷϡҺѪ

ԌգCCPԌռУƷϡҺѪ

A᣻^

BҶcTMB

KֹҺ 

ϴҺ Na2HPO4.12H2ONaClKClKH2PO4œ-20

ҪMɣ

1

CCPԭ

1K 96˷

 

1K48˷

2

øY(ji)

1ƿ10ml

 

1ƿ5ml

3

Ԍ

1ƿ1ml

 

1ƿ1ml

4

Ԍ

1ƿ1ml

 

1ƿ1ml

5

ϴҺǰ120ϡጣ

1ƿ50ml

 

1ƿ50ml

6

 A

1ƿ6ml

 

1ƿ3ml

7

B

1ƿ6ml

 

1ƿ3ml

8

KֹҺ

1ƿ6ml

 

1ƿ3ml

9

ƷϡҺ

2ƿ50ml

 

1ƿ50ml

10

1ƿ1ml

 

1ƿ1ml

11-15

УƷϵ

5ƿ*1ml

 

5ƿ*1ml

16

f

1

 

1

17

3

 

3


lЧڡ

l2-8汣Чڃ(ni)ʹ

Чڣ12 (g)

a(chn)ڼʧЧҊ(bio)


mÃx

Ԅ(dng)ø(bio)yxL450nmL630nm


ӱҪ

ѪѪ{o}Ѫxī@ܷ(f),(bio)2-8ɱһ


z(yn)

1ÿԇ(yn)O(sh)հ׌һףӘƷø(bio)ԇͬԌԌԌոɿzyr(sh)߀O(sh)УƷ5(g)ȸһyӱØƷϡҺ1100ϡጺ100l΢װ3760犡ȥ׃(ni)Ʒ۸ɡ

2ϴҺעMÿף400lϴҺ/ףo5犺۸؏(f)5

3ÿ׷քe100løY(ji)Ҝط30ͬϷϴ(yng)5Ρ

4ÿ׼@ɫAҺBҺ50lppʎ37ܹo15

5ÿ׼KֹҺ50lKֹ(yng)Կհ{(dio)ø(bio)xxȡOD450


z(yn)Y(ji)Ľጡ

1 ڶԜy

(1) Y(ji)Ӌ(j)㣺yֵպ͘Ʒpȥհ׌ֵȻӋ(j)㌦պ͘ƷĽ^ȱֵ

                     ջƷֵ(OD450)

ȱֵ=-----------------------------

                     Ԍֵ(OD450)


2Y(ji)Дࣺ

ȱֵ

Y(ji)

 

< 0.95

 

0.95,1.0

߅ֵhœy

 

>1.0


2ڶ\ࣺ

1Y(ji)Ӌ(j)㣺5(g)УƷУƷA 100 RU/mlУƷB 50 RU/mlУƷC 25 RU/mlУƷD 12 RU/mlУƷE 6 RU/mlȣLog(sh)X-SȣY-S(bio)(zhn)(j)Ѫп-ccpwĝӋ(j)C(j)MϷʽLƘ(bio)(zhn)ą(sh)(sh)兢(sh)(sh)splineM(sh)/(sh)lim/limitѪӱȳУƷA100RU/ml,\Y(ji)Ɉ(bo)飾100RU/mlͬr(sh)hxһ(g)m(dng)?sh)ϡጱ?sh)Ѫϡጺٜy1400Ę(bio)(zhn)Ӌ(j)Ĕ(sh)(j)4ӱccpw

2Y(ji)Дࣺ12RU/ml

12RU/ml

z(yn)ľԡ

ƷHwzyѪѪ{пh(hun)ϰĿw(CCP)L(fng)P(gun)(ji)ײ˵o\


a(chn)Ʒָ(bio)

1ﰲȫԣƂԌƷԌƷԌƷѪ轛(jng)ﰲȫԙzy͸ײ濹ԭzy͸ײwzyȱݲHIV-1/2wzy

2ԇ^ҺwM֑(yng)Һw

3bԇbݘ(bio)ʾb

4ԇհȣ0.05

5ԇ`ȣyԇЃ(ni)У(zhn)ҺEஔ(dng)6RU/mlȷ0.06-0.16

6(zhn)_ȣyԇЃ(ni)У(zhn)ҺBஔ(dng)50RU/mlȷ1.0-2.0

7Է6100RU/ml(ni)Ӌ(j)-(yng)P(gun)ϵ(sh)r0.95

8ԅƷʣ10ԅƷy(yng)ȫz

9ԅƷʣ10ԅƷy(yng)ȫz

10ԣþԅƷy,CV15n=10

11(wn)ԇ(yn)3762-812(g)™zY(ji)(yng)_(d)2410(bio)(zhn)

ע(xing)

1sϴҺʹǰsˮϡ20ʹ

2̖ͬԇɻʹ

3횇(yn)Ɯ(yng)ĜضȺ͕r(sh)g

4Y(ji)жԃxy(zhn)ܑ{^

5(dng)ԌODֵ1.80ԌOD0.12r(sh)(sh)(yn)Y(ji)Чġ

t(y)еa(chn)I(y)SC̖ʳˎO(jin)еa(chn)S20030955̖

t(y)еעC̖/a(chn)Ʒg(sh)Ҫ̖

f˜(zhn)ڼ޸ڡ