ˎƷQ(chng)
ͨQ(chng)ײ濹ԭ\ԇУø(lin)߷
ӢQ(chng)Diagnostic Kit for Hepatitis B Virus Surface Antigen (ELISA)
hZ(y)ƴYixing Ganyan Bingdu Biaomian Kangyuan Zhenduan Shijihe (Meilianmianyifa)
bҎ(gu)
48˷/С96˷/576˷/
A(y);
ײ濹ԭ\ԇmڶԜy(c)ѪѪ{ײ濹ԭѪҺY鼰RײȾo\
z(yn)ԭ
ײ濹ԭ\ԇвpwAķzy(c)HBsAgÿ-HBslHRP(bio)ӛĿ-HBsø(bio)ӛļ(lin)TMB^(gu)(dng)(bio)дHBsAgr(sh)ԓHBsAgc-HBsY(ji)ϲc-HBs-HRPY(ji)γɿ-HBs-HBsAg--HBs-HRP(f)TMBa(chn)@ɫ(yng)֮to(w)@ɫ(yng)ڌ(sh)(yn)Y(ji)r(sh)ɫ׃ʾHBsAgڣo(w)ɫɫ׃tʾHBsAg
ҪMɳɷݡ
ԇнMɣ |
48˷/ |
96˷/ |
576˷/ |
|||
1.A(y)(yng) |
48/K |
1K |
96/K |
1K |
96/K |
6K |
2.øY(ji) |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
3.(yng)Ԍ(du) |
0.8ml |
1ƿ |
0.8ml |
1ƿ |
4ml |
1ƿ |
4.Ԍ(du) |
0.8ml |
1ƿ |
0.8ml |
1ƿ |
4ml |
1ƿ |
5.sϴҺ |
30ml |
1ƿ |
30ml |
1ƿ |
100ml |
2ƿ |
6.@ɫA |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
7.@ɫB |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
8KֹҺ2MH2SO4 |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
9.z |
2Ƭ |
2Ƭ |
12Ƭ |
|||
10.f(shu)(sh) |
1 |
1 |
1 |
|||
11.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬ(ho)ԇԇнM12 ɻ
Ʒ̓x
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌ(j)r(sh)
7. ϴC(j)ϴƿ
8. ʎ
9. ø(bio)x zy(c)L(zhng)450nmL(zhng)630nm
(ch)lЧڡ
ԇБ(yng)28бܹⱣЧ12(g)¡
ӱҪ
1. ӱɼҪ(zhn)Ҫܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2Сr(sh)ٌѪƷx3000D(zhun)/x6ʹѪ壨{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr(sh)
2. ӱʹѪѪ{ػEDTAȿ(hu)Ӱ푌(sh)(yn)Y(ji)
3. ӱкïBc(hu)Ӱ푌(sh)(yn)ĽY(ji)߶ѪĘӱ](mi)ȫտsѪӱȾĘӱܕ(hu)e(cu)`ĽY(ji)
4. rĘӱ28o(w)lɱһrӱL(zhng)A-20£ⷴ(f)
z(yn)
1. (sh)(yn)(zhn)䣺حh(hun)ȡԇҜƽ30ͬr(sh)sϴҺ120ϡ
2. Ӵy(c)(bio)ÿԇ(yn)O(sh)(yng)Ԍ(du)ոɿքeꎡ(yng)Ԍ(du)0.1հ(du)1y(c)(bio)0.1, 3760犡
3. øY(ji)ÿ0.05հ(du)տײֻ3730
4. ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעM(mn)ÿףo5-10ȥ׃(ni)ϴҺĸ˷(f)5ĸɡ
2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עM(mn)ÿ_ÿo(w)ˮĸ
5. @ɫ ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ30
6. Kֹ(yng) ÿKֹҺ0.05
7. y(c)ø(bio)xx(sh)xβL(zhng)450 nmԿհУ㣩pL(zhng)450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)
ֵ
1. Ԍ(du)ƽֵС0.1(yng)Ԍ(du)ƽֵ1.2(sh)(yn)Y(ji)Ч
2. Ԍ(du)ODֵ0.050.05Ӌ(j)0.05(sh)HODֵӋ(j)
3. Cutoff ֵӋ(j)㣺 COV = Ԍ(du)ƽODֵ2.1
z(yn)Y(ji)Ľጡ
1. (bio)ODֵCOV(yng)(bio)ODֵCOV
2. ԽY(ji)ӱвHBsAgӱеHBsAgԇеęzy(c)
3. (yng)ԽY(ji)ӱкHBsAgخ(yng)
4. (yng)ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)ֻн(jng)^(gu)؏(f)؏(f)(sh)(yn)Ԟ(yng)ԽY(ji)ĘӱJ(rn)HBsAg(yng)؏(f)Y(ji)(yng)ԵĘӱ(yng)(jng)^(gu)m(dng)?sh)ֶM(jn)д_J(rn)
(yng)ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)(yng)ԽY(ji)܌һ(g)(g)g(sh)(wn)}£
1)ڃxӘɵĽȾ
2)@ɫxȾ
3)ԇ©ɵĽȾ
4)ֵϴȥصס
z(yn)ľԡ
иԵߌ(sh)(yn)ϵy(tng)ڝڵķخԷ(yng)؏(f)(yng)ԽY(ji)(yng)һ(g)m(dng)?sh)ķM(jn)д_J(rn)
a(chn)Ʒָ(bio)
1. ԇ^(gun)ҺwM֑(yng)
2. ԅƷʣÇ(gu)҅ƷM(jn)ЙzóF(xin)(yng)
3. (yng)ԅƷʣÇ(gu)҅ƷM(jn)Йzzy(c)3ݝֵ5104IU/mlHBsAg(yng)ԅƷóF(xin)
4. ͙zÇ(gu)҅ƷM(jn)ЙzHBsAg adradway͵͙zҪ
5. ԣþԅƷy(c)CV%(yng)15%n=10
6. (wn)ԇ(yn)ԇM376zY(ji)_(d)Ϙ(bio)(zhn)
7. bݘ(bio)ʾb
ע(xing)
1. z(yn)ϴ|(zh)P(gun)ҪȱϴҺ^(gu)ܳM(mn)(yng)ϴΔ(sh)(yng)5(jng)עzҺ^Ƿ
2. ֹϴr(sh)Ո(qng)ʹÎмˮİ壬ԷԴ^(gu)ø(li)߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zy(c)Y(ji)Ĝ(zhn)_ԡ
3. ϴr(sh)õˮՈ(qng)(f)ʹ
4. ϴC(j)ÿʹǰsˮȥxˮ_ϴɃԷֹ·g
5. κԇ|Ƥw۾ôˮ(du)ԓλM(jn)ДU(ku)ϴKֹҺиg
6. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈ(qng)P(gun)Č(sh)(yn)ҹҎ(gu)(li)(zh)к̎
7. ʹȫԄ(dng)øϵy(tng)ܕ(hu)ϵy(tng)`(yng)(du)ϵy(tng)(sh)O(sh)m(dng){(dio)ԝM(mn)(gu)P(gun)|(zh)ƷҪ
ˎƷa(chn)SC̖(ho):20160052
a(chn)Ʒ(zhn)̖(ho)(gu)ˎ(zhn)S10950045
a(chn)Ʒ(bio)(zhn)̖(ho)YBS00412010
f(shu)(sh)(zhn)ڡ